骨龄自动化评估的研究进展

骨龄是反映人体生长发育的一项重要指标,能够较为客观地反映个体生长发育水平及成熟度。传统的人工骨龄评估通常是将左手腕X线片与参考标准进行比较,从而获得相应的骨龄值,该方法既耗时又存在观察者间的差异。近年来,随着计算机科学的不断发展,促使骨龄评估开始由传统的人工评估向自动化评估转变。虽然骨龄自动化评估的研究已相当多,但大部分仍处于实验阶段。本文综述了近年来国内外学者在骨龄自动化评估方面的相关研究与进展,以期为相关研究人员提供参考与研究思路。     

单一大关节判定男性青少年骨龄可靠性的比较研究

通过男性青少年单一关节X线片判定年龄是快速、简便的一种方法,但这些方法哪一种更准确尚无系统研究。本文研究了100例正常男性青少年X线片(13~18岁)对单一大关节判断年龄的结果进行了初步比较,结果发现在不同年龄段每种方法的准确性是不同的。在15~16岁之前,百分位数法、CHN法、肩关节法、肘关节法和腕关节法准确性更高些。在15~16岁之后,六大关节法、膝关节法、踝关节法和髋关节法的准确性更好。     

骨骼个体识别系统的研究

骨骼个体识别系统的研究花锋，田雪梅，孙建军，沈旭昆ＳｔｕｄｙｏｆｔｈｅＣｏｍｐｕｔｅｒＳｙｓｔｅｍＢａｓｅｄｏｆｔｈｅＩｎｄｉｖｉｄｕａｌＩｄｅｎｔｉｆｉｃａｔｉｏｎｏｆＳｋｅｌｅｔｏｎ￥Ｈｕａｆｅｎｇ；Ｔｉａｎｘｖｅｍｅｉ；Ｓｕｎｊｉａｎｊｕｎ；（...     

The squatting facet on femora of West Coastal Indian population

104 out of 108 adult femora from the West Coastal region of India show a distinct identifiable squatting facet on their popliteal surface. The data on such features are reviewed and its relevance to medico legal and anthropological science is discussed.     

用chelex-100提取骨骼DNA的研究

建立了应用chelex-100加蛋白酶K提取骨骼DNA的方法,并对1～30年31例骨骼进行了实验.该方法操作简单、快速、重复性好,灵敏度以及阳性率高,适用于法医学鉴定.     

Ossification Degrees of Cranial Sutures Determined with Flat‐Panel Computed Tomography: Narrowing the Age Estimate with Extrema

Abstract: Since Broca’s time (1824–1880), ossification of the neurocranial sutures has been used as a characteristic of age. Current approaches include the visual macroscopic examination of ecto and endocranial sutures. The evaluation of the cross‐section of sutures usually necessitates the destruction of the neurocranium. In a nondestructive alternative approach that was tested within the context of the “Digital Forensic Osteology” project that ran in cooperation with the Virtopsy^®‐Project, it emerged that the resolution of conventional multi‐slice computed tomography data sets was not high enough to image sutures. Thus for the experiments presented here, the eXplore Locus Ultra flat‐panel computed tomography scanner from GE Healthcare was used. Calottes were scanned during autopsy and then immediately returned to the corpse. So far, the skullcaps of 221 individuals have been scanned. The cross‐sections of 14 suture segments could be assessed for seven previously defined stages of ossification. In a converse step, the 14 highest and lowest age estimate values corresponding to the individual stages of suture closure found were estimated for each calotte. The obtained ranges narrowing down the age estimate were evaluated with statistics. A mean value of 43.31 years for the range of narrowed age estimates shows that this method can be a useful aid in estimating age. The results of intra‐ and inter‐observer tests showed good overall agreement between the findings of three observers. This method is suitable for a nondestructive age estimation and can be used for the entire calotte.     

Assessing zygomatic shape and size for estimating sex and ancestry in a South African sample

Unidentified, decomposed and skeletonised human remains are frequently found in South Africa, therefore, standardised, reliable and relevant sex and ancestry estimation methods are required for forensic identification. This study assessed sex and ancestral variation in zygomatic size and shape in a South African population using geometric morphometric analyses. The zygoma of 158 South African individuals were sampled. Eight zygomatic landmarks were captured in 3-dimensions using a Microscribe G2 digitiser and assessed using procrustean geometric morphometrics. Shape and size differences were analysed using multivariate linear regression, discriminant function and canonical variate analyses. Males had significantly larger zygomas than females. Significant shape variation was found between ancestral groups. Bantu-speaking and Mixed ancestry individuals had narrower, shorter and more anteriorly projecting orbital margins, whilst Europeans had vertically elongated and receded orbital margins. European ancestral groups were most discernible from Bantu-speakers and Mixed ancestral groups. Ancestry estimation accuracies improved when ancestry was aggregated with sex. Pairwise ancestry-linked comparisons in females were as follows; Bantu-speakers (76%) from Europeans (72%), Bantu-speakers (71%) from Mixed ancestry (59%) and European (72%) from Mixed ancestry (63%). Similarly, ancestry-linked comparisons in males were as follows; Bantu-speakers (77%) from Europeans (81%), Bantu-speakers (53%) from Mixed ancestry (59%) and European (72%) from Mixed ancestry (82%). Size differences are putatively linked to variations in hormone-regulated growth and muscular robusticity between males and females. Shape variations between ancestral groups are likely attributable to the heterogenous genetic and ancestral origins of the South African population. It is challenging to distinguish between South Africa Bantu speakers and Mixed ancestry people due to Mixed ancestry individuals having variable genetic contributions from Khoesan, Bantu-speakers, Europeans and Asians. Bantu-speaking and Mixed ancestry people had zygomatic morphologies consistent with historical thermoregulatory adaptations to sub-Saharan climates, reported in African-descendants. Zygomatic morphology in European descendants suggests ancestral origins from colder climatic regions. This study demonstrated the utility of the zygoma in distinguishing between ancestral groups in South Africa, but further research is required to develop population-specific standards to distinguish between South African populations with shared African ancestry. The zygoma shows a promising ability to estimate sex and ancestry in South Africans, suggesting population specific standards for this bone may be of forensic interest.     

一种改良骨骼DNA的提取方法

采用十六烷基三甲基溴化铵(简称CTAB)及两次纯化浓缩的方法提取骨骼DNA,利用荧光标记复合扩增、自动毛细管电泳与分型的技术,成功检测到14个STR位点的分型.该方法稳定、结果可靠,为严重降解骨骼DNA的提取提供了一种可参用的方法.     

Development of Multiple Assays using 46 SNPs for Comprehensive mtDNA Haplogrouping and Application to Highly Degraded DNA

Six multiplex PCR systems using single‐base extension reactions to analyze 46 mitochondrial DNA (mtDNA)‐coding region single nucleotide polymorphisms (SNPs) that define 42 haplogroups, that is, 24 major mtDNA haplogroups and 18 subclades, were devised. To improve the usefulness of the established systems for the analysis of degraded DNA samples, novel primers to render amplicons with sizes <150 bp were designed. By applying these systems to 214 Japanese individuals, 24 different haplogroups (power of discrimination = 93.4%) were found. To assess the effectiveness of our systems in grouping degraded DNA, an ancient bone sample of a Jomon skeleton was analyzed and then classified as haplogroup N9b. We conclude that the present systems are powerful screening tools for major haplogroups of mtDNA in addition to the prevalent subhaplogroups in the Japanese population and that these systems are capable of analyzing highly degraded DNA samples in forensic studies.