| 应用Dot-ELISA检测牛胴体沙门菌 |
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| 引用本文： | 张红见,韩志辉,魏建华,扎西英派.应用Dot-ELISA检测牛胴体沙门菌[J].中国兽医科学,2004,34(2):54-56. |
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| 作者姓名： | 张红见 韩志辉 魏建华 扎西英派 |
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| 作者单位： | 1. 青海大学,农牧学院,青海,西宁,810003
2. 青海省畜牧兽医科技咨询站,青海,西宁,810008 |
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| 摘 要： | 应用Dot ELISA法对西宁市某屠宰点 71份牛胴体淋巴结进行了沙门菌检测 ,同时与常规分离培养鉴定技术比较。结果 ,在 71份牛胴体样本中 ,用Dot ELISA法检出沙门菌阳性 33份 ,阳性率为 4 6 .4 8% (33/71) ;而用常规分离培养鉴定技术检出沙门菌阳性 31份 ,阳性率为 4 3.6 6 %(31/71) ;2种方法的阳性符合率为 81.82 % ,阳性检出率差异不显著 (P >0 .0 5 )。
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| 关 键 词： | Dot-ELISA 检测 牛胴体 沙门菌 |
| 文章编号： | 1000-6419(2004)02-0054-03 |
| 修稿时间： | 2003年6月10日 |
Detection of Salmonella in carcasses of beef by Dot-ELISA |
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| ZHANG Hong-jian,HAN Zhi-hui,WEI Jian-hua,ZHAXI Ying-pai.Detection of Salmonella in carcasses of beef by Dot-ELISA[J].Veterinary Science in China,2004,34(2):54-56. |
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| Authors: | ZHANG Hong-jian HAN Zhi-hui WEI Jian-hua ZHAXI Ying-pai |
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| Institution: | ZHANG Hong-jian~1,HAN Zhi-hui~1,WEI Jian-hua~2,ZHAXI Ying-pai~1 |
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| Abstract: | Salmonella in 71 lymphglands of beef carcass was detected by Dot-ELISA in slaughter (houses.) At the same time, the technology of routine isolation culture was chosen as control method. The (result) showed that Salmonella was found in 33 out of 71 lymphglands by Dot-ELISA i.e. positive rate was 46.48%, and Salmonella was found in 31 out of 71 lymphglands by the technology of routine isolation (culture) and there was no difference between by Dot-ELISA and by the technology of routine isolation (culture) for detection of Salmonella in carcasses of beef. |
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| Keywords: | Dot-ELISA Salmonella beef carcass detection |
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