软肝饮对免疫性肝纤维化大鼠胶原代谢水平的影响

目的：建立大鼠免疫损伤性肝纤维化的实验模型，研究中药复方软肝饮对血清层粘；连蛋白(Laminin，LN)、透明质酸(Hyaluronic acid，HA)、血清Ⅲ型前胶原(Precollagen typeⅢ，PCⅢ)、肝脏羟脯氨酸(Hydroxyproline，Hyp)含量的影响，探讨其抗肝纤维化的作用机制。方法：在以人血白蛋白(HSA)诱导的免疫损伤性肝纤维化大鼠模型基础上，软肝饮小剂量(1g／kg)，大剂量(2g／kg)灌胃给药，连续8周，以鳖甲软肝片和秋水仙碱为阳性对照药；放射性免疫方法测定大鼠血清中HA、LN、PCⅢ含量；化学法测定肝脏羟脯氨酸含量。结果：软肝饮两个剂量组可明显降低HSA诱导免疫性肝纤维化大鼠模型血清中HA、LN、PCⅢ的含量，同时可降低大鼠肝脏中羟脯氨酸的含量。结论：软肝饮通过对肝脏胶原物质代谢的影响而产生抗肝纤维化的治疗作用。

Effect of Ruangan-Yin on Level of the Collagen Metabolism in Rats with Immunity Liver Fibrosis

Objective:The rat model with immunity liver fibrosis was established to study the effect of composite herbal medicines of Ruangan-Yin on the levels of serum laminin, hyaluronic, precollagen type III, and the level of hydroxyproline in liver. So the mechanism of anti-fibrosis was explored. Method: The rat models with immunity liver fibrosis were induced by the human serum albumin, Ruangan-Yin were took by gastric infusion respectively as little and large dosage group(1 g/kg and 2 g/kg) in consecutive 8 weeks. Biejia Ruangan Tablet or colchicines were took as positive contrast drug. The levels of serum laminin, hyaluronic, precollagen type III were detected by radioimmunoassay. The level of hydroxyproline in liver was detected by chemistry method. Result: Ruangan-Yin could depress the serum levels of laminin, hyaluronic, precollagen type III in rat models with immunity liver fibrosis induced by the human serum albumin in two dosage groups. At the same time, it depressed the level of hydroxyproline in rat liver. Conclusion: Ruangan-Yin could affect the collagen metabolism in liver to treat liver fibrosis.