华蟾素配伍丝裂霉素体外抑瘤作用

目的：从体外药敏实验的角度观察华蟾素注射液配伍丝裂霉素对不同肝癌细胞抑瘤效果和对正常肝细胞的毒性反应。方法：通过四甲基偶氮唑盐比色法观察低浓度华蟾素注射液、丝裂霉素及两配伍使用时对人肝癌细胞系SMMC-721、BEL—7402细胞及正常肝细胞系L-02细胞的生长抑制作用。结果：对人肝癌细胞的生长抑制作用，华蟾素及丝裂霉素两配伍使用较两单独使用显增强；丝裂霉素对正常肝细胞具有一定生长抑制作用，而华蟾素注射液则无此作用，与单独使用丝裂霉素比较，两的配伍使用对正常肝细胞的生长抑制作用未见明显增加。结论：华蟾素和丝裂霉素配伍使用可显提高对人肝癌细胞的杀伤作用，同时未增加对正常肝细胞的生长抑制作用，提示华蟾素和丝裂霉素配伍使用在肝癌治疗中具有很好的临床应用前景。

Anti-tumor Effect of Cooperation of Cinobufacine Injection with Mitomyci C in vitro

Objective:To investigate the anti tumor effects of cooperation of cinobufacine injection with mitomycin C in vitro.Method: The cytotoxicity of mitomycin C cooperated with cinobufacine injection against established hepato cell lines(SMMC 7721,BEL 7402 and L 02 cell)was determined by MTT assays. Mitomycin C, cinobufacine injection were used separately as control. Result: Cooperation of cinobufacine injection with mitomycin C showed much more better anti tumor effect than the drugs used separately, but do not significantly enhance the growth inhibitory rate of normal hepatic cell lines. Conclusion: Cooperation of cinobufacine injection with mitomycin C has better anti tumor effect than the drugs used separately,meanwhile it do not enhance cytotoxicity to normal hepatic cell line, showing its feasibility and validity in hepatocellular carcinoma curing.