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| D3S1358等7个DNA位点的荧光法在法医学中的应用 | |
| 引用本文： | Que T. D3S1358等7个DNA位点的荧光法在法医学中的应用[J]. 法医学杂志, 2000, 16(1): 14-15 |
| 作者姓名： | Que T |
| 作者单位： | 司法部司法鉴定科学技术研究所,上海,200063 |
| 摘 要： | 报道了D3S1358、D16S539等7个DNA位点的复合扩增和四色荧光分析技术。经310自动测序仪检测7个位点 ,实现了STR位点同步扩增和自动检测目的。同一性实验表明 ,同一个体肌肉、血液、唾液和头发等组织STR位点基因型完全一致。对2个四代家系、3个三代家系的调查结果表明 ,这些位点符合孟德尔遗传定律。D3S1358、D16S539等7个位点对陈旧、微量的检材适用性很强,灵敏度达75pg ,在法医个人识别和亲子鉴定中有着重要意义 |
| 关 键 词： | D3S1358 D16S539 TH01 TPOX CSF1PO D7S820 Amelogenin 荧光检测 |
| 修稿时间： | 1999－09-25) |
Multiplex PCR fluorescent detection of DNA loci and its application in forensic science |
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| Que T. Multiplex PCR fluorescent detection of DNA loci and its application in forensic science[J]. Journal of Forensic Medicine, 2000, 16(1): 14-15 | |
| Authors: | Que T |
| Affiliation: | Institute of Forensic Sciences, Ministry of Justice, Shanghai 200063, P. R. China. |
| Abstract: | A method is reported using PCR multiplex amplification and fluorescent-labeled primers to genotype D3S1358, D16S539, TH01, TPOX, CSF1PO, D7S820 and Amelogenin loci. It is simple, accurate, sensitive and very useful for personal identification and paternity testing. |
| Keywords: | D3S1358 D16S539 TH01 TPOX CSF1PO D7S820 amelogenin fluorescence detection |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ | |