| 用醛化红细胞分离抗禽流感病毒独特型多克隆抗体 |
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| 引用本文： | 李宝全,彭军,尹逊河,高强,牛钟相.用醛化红细胞分离抗禽流感病毒独特型多克隆抗体[J].中国兽医科学,2005,35(7):512-514. |
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| 作者姓名： | 李宝全 彭军 尹逊河 高强 牛钟相 |
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| 作者单位： | 山东农业大学,动物科技学院,山东,泰安,271018 |
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| 摘 要： | 用纯化的鸡抗禽流感病毒(AIV)抗体免疫家兔,制备抗独特型多克隆抗体;用戊二醛醛化的红细胞固定非特异性鸡抗体,再吸附兔抗鸡抗体,获得了纯的抗独特型抗体。经鉴定,该抗体既有弱的血凝性又有弱的血凝抑制性,并能与原病毒竞争性地和鸡抗AIV抗体结合。
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| 关 键 词： | 禽流感病毒 抗独特型抗体 醛化红细胞 |
| 文章编号： | 1000-6419(2005)07-0512-03 |
| 修稿时间： | 2005年1月4日 |
Isolation of polyclonal anti-idiotypic antibodies to AIV by aldehydated erythrocyte |
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| LI Bao-Quan,PENG Jun,YIN Xun-he,GAO Qiang,NIU Zhong-xiang.Isolation of polyclonal anti-idiotypic antibodies to AIV by aldehydated erythrocyte[J].Veterinary Science in China,2005,35(7):512-514. |
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| Authors: | LI Bao-Quan PENG Jun YIN Xun-he GAO Qiang NIU Zhong-xiang |
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| Abstract: | Polyclonal anti-idiotypic antibodies to AIV were prepared from rabbits vaccinated with purified chicken anti-AIV antibodies, and isolated by aldehydated chicken red blood cells to bind unspecific chicken antibodies and to absorb rabbit-anti-chicken antibodies. The acquired antibodies were capable of agglutinating chicken red blood cells and inhibiting AIV in hemagglutinating weekly, and competed original AIV in binding chicken anti-AIV antibodies. |
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| Keywords: | AIV anti-idiotypic antibody aldehydated erythrocyte |
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