尿中氯喹定性定量分析方法的研究

建立了人尿中氯喹的定性定量分析方法,2ml尿样用2ml×2环己烷：乙酸乙酯（8：2）提取净化后,60℃水浴室气吹干,残留物定容溶解后,气相色谱分析,氯喹的保留时间为9．44min。方法最低检测限为200ng／ml,回收率为87．0％,RSD＝7．9％（n＝5）,在0～50μg／ml浓度范围内,有良好的线性关系：A＝1778．9＋13686C,r＝0．999。方法同时可用于血中氯喹的分析。附一例应用报告,测得尿中氯喹的含量为0．745mg／ml,血中氯喹的含量为3．68μg／ml。尿液中同时检出氯喹的N－去单已基代谢物。定性结果经质谱法验证。

Determination of chloroquine in human urine

A GC method was established for qualitative and quantitative determination of chloroquine in human urine. The chloroquine is extracted from 2 ml urine with 2 x 2 ml cyclohexame: chloroform (8:2). The retention time of chlorquine is 9.44 min. The linear of quantitation is 0-50 micrograms/ml. The recovery of the procedure in 25 micrograms g/ml is 87.0%, CV = 7. 9% (n = 5). The limit of the determination is 200 ng/ml. The method can also be used in blood. A case is reported here. In that, we detected chloroquine in urine and blood. The quantitation of chloroquine in urine and blood is 0.475 mg/ml and 3.68 micrograms/ml, respectively. A metabolite of chloroquine-desethylchloroquine is also found in the urine.