APM染色法在羊水栓塞诊断中的应用价值

目的　探讨APM染色法在羊水栓塞 (AFE)病理学诊断中的应用价值。方法　采用APM染色法对 1988年至 2 0 0 1年确诊为AFE的 19例病例 (AFE组 )和羊水吸入性肺炎的胎儿 3例 (阳性对照组 )、其它死因的 10例产妇 (阴性对照组 )重新进行染色和组织学检查 ,对其检验结果进行比较分析。结果　阳性组全部检出羊水成分 ,阴性组部分病例检出羊水成分 ,发现漏诊、误诊各 1例。与HE染色相比较 ,APM染色能提高角化上皮和粘液的检出率。结论　APM染色能提高羊水成分的检出率 ,有助于提高AFE诊断的准确性 ,可用于AFE病理学诊断。

The value of APM histochemical stain technique in the diagnosis of amniotic fluid embolism

Objective Explore the value of APM histochemical stain technique in the diagnosis of amniotic fluid embolism(AFE).Method The pulmonary tissues taken from autopsies performed in 1988～2001 including 19 AFE cases and 3 babies died of amniotic fluid aspiratory pneumonia(positive control),10 women in labor died of other causes(negative control) were sectioned again and stained by APM method and HE method. Comparative statistical research was carried out on findings from the 2 staining methods. Results By APM stain,amniotic fluid elements could be found in all AFE cases and positive control cases as well as some negative control cases.In addition,we found 1 positive case neglected and 1 false negative case of AFE retrospectively.Statistical comparison revealed that APM stain can elevate the detection rates both for keratinized epithelium and mucus.Conclusion APM staining provided possibility of detecting some amniotic fluid elements that was not distinguished by HE stain. The APM stain is helpful and can be used for AFE diagnosis in routine histological examination.