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| 改良IPEP法用于痕量DNA检测的实验研究 | |
| 引用本文： | 陈玲,刘超,杨电,李越,胡慧英,王慧君.改良IPEP法用于痕量DNA检测的实验研究[J].中国法医学杂志,2007,22(1):4-7. |
| 作者姓名： | 陈玲 刘超 杨电 李越 胡慧英 王慧君 |
| 作者单位： | 1. 南方医科大学法医学研究所,广东,广州,510515 2. 南方医科大学法医学研究所,广东,广州,510515;广州市刑事科学技术研究所,广东,广州,510030 3. 广州市刑事科学技术研究所,广东,广州,510030 |
| 基金项目： | 公安部应用创新计划;广东省科技厅科技计划;广东省广州市科技攻关项目 |
| 摘 要： | 目的探讨改良扩增前引物延伸(IPEP)法对痕量DNA样本STR检测分型的效果。方法用改良IPEP法对痕量样品DNA进行全基因组扩增(WGA),扩增产物用实时荧光定量PCR技术定量、用AmpFLSTR~ Indentifiler~试剂盒作基因型检测。结果该方法可增加模板DNA约200~1100倍。基因组DNA不低于0.025ng时,可获得15个STR基因座和Amelogenin性别基因座的分型结果。基因组DNA0.01~0.025ng时,可获得9个以上基因座的分型结果。结论改良IPEP法可有效提高痕量DNA样本STR分型检验的灵敏度,有较好的实用价值。 |
| 关 键 词： | 法医物证学 改良IPEP 全基因组扩增 痕量DNA检测 |
| 文章编号： | 1001-5728(2007)01-0004-04 |
| 修稿时间： | 2006年2月22日 |
Study on application of modified IPEP in trace DNA analysis |
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| Abstract: | Objective To study the effect of modified improved primer extension preamplification (IPEP) on STR analysis of trace DNA.Methods Whole genome amplification (WGA) was performed for trace DNA using modified IPEP, and the amplified products were quantitated by real-time quantitative PCR as well as genotyped by AmpFLSTR~ Indentifiler~ kit.Results The template DNA can be amplified 200 to 1100 folds by modified IPEP. Using this technique, all 15 STR loci and amelogenin gene can be genotyped correctly when the genomic DNA is more than 0.025ng, and more than 9 STR loci can be genotype with success while the genomic DNA is between 0.01ng to 0.025ng.Conclusion Modified IPEP can increase the sensitivity of STR analysis and is helpful for trace DNA analysis. |
| Keywords: | Forensic biological evidence Modified IPEP Whole genome amplification Trace DNA analysis |
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