Multiplexing of Y chromosome specific STRs and performance for mixed samples |
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| Institution: | 1. Department of Radiation Oncology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania;2. Currently at Department of Radiation Oncology, Columbia University Medical Center, New York, New York;3. Department of Biostatistics and Epidemiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania;4. Currently at Biostatistics and Bioinformatics Facility, Fox Chase Cancer Research Center, Philadelphia, Pennsylvania;5. Currently at Department of Radiation Oncology, Brigham and Women’s Hospital/Dana Farber Cancer Institute, Boston, Massachusetts;6. Department of Internal Medicine, Division of Hematology and Medical Oncology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania;7. Currently at Department of Radiation Oncology, University of Washington School of Medicine, Seattle, Washington |
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| Abstract: | A combination of four Y-specific polymorphic STR loci was amplified simultaneously using fluorescently labeled primers. Multiplex conditions required optimization to eliminate constant bands and amplification products for female DNA. A series of experiments was carried out for mixtures of DNA from two males, and from male and female individuals for the Y-specific STRs and an autosomal locus. For the male/male mixtures amplified with the Y specific system, and amplified for an autosomal locus, the minor component in the mixture could only be identified up to a ratio of 1:10, 1:50 respectively. In male/female DNA mixtures the Y STR alleles could be identified for the highest ratio tested, 400 pg male in DNA in 800 ng female DNA which amounts to a ratio of 1:2000. |
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