| 单个细胞DNA样本的制备及其在灵敏度分析中的应用研究 |
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| 作者姓名： | Deng JQ Shi MS Ying BW |
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| 作者单位： | 1. 司法部司法鉴定科学技术研究所,上海,200063
2. 四川大学华西基础医学与法医学院,四川,成都,610041 |
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| 基金项目： | 国家自然科学基金资助项目(30271446),高校博士点专项科研基金资助项目(20020610044) |
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| 摘 要： | 目的建立一种用于高精确灵敏度分析的微量DNA样本制备方法。方法用显微捕获技术制备以细胞为单位的DNA样本,并用于ProfilerPlus试剂盒及ABI310遗传分析仪的灵敏度测试。结果建立了单细胞捕获操作方案,成功制备了依次含1～11个细胞的一组DNA分析样本,并可准确用于ProfilerPlus誖试剂盒及ABI310遗传分析仪的灵敏度研究。结论基于显微操作技术的高精确微量DNA分析样本制备方法可准确用于分析技术方法的灵敏度研究
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| 关 键 词： | 灵敏度分析 DNA样本制备 单个细胞 |
| 文章编号： | 1004-5619(2005)01-0006-03 |
| 修稿时间： | 2004年11月8日 |
DNA samples preparation from single cell and its application in sensitivity test |
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| Deng JQ,Shi MS,Ying BW.DNA samples preparation from single cell and its application in sensitivity test[J].Journal of Forensic Medicine,2005,21(1):6-8. |
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| Authors: | Deng Jian-qiang Shi Mei-sen Ying Bing-wu |
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| Institution: | Institute of Forensic Science, Ministry of Justice, PR China, Shanghai 200063, China, |
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| Abstract: | OBJECTIVE: To establish a reliable, exact and practical method to prepare DNA samples for sensitivity-test purposes. METHODS: The micromanipulation method was employed to prepare exact quantity DNA samples used to study the sensitivity of Profiler Plus Kit-ABI PRISM 310 system. RESULTS :We succeed in establishing a micromanipulation method to prepare groups of DNA samples, which contain 1-11 cells in turn, and also succeed in using them to study the sensitivity of Profiler Plus Kit-ABI310 system. CONCLUSION: The method we have established is proved to be a reliable, exact and practical way to prepare DNA samples for sensitivity-test purposes. |
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| Keywords: | sensitivity test DNA sample preparation single cell |
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