| 负载灭活口蹄疫病毒树突状细胞启动的CD4~+T细胞应答 |
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| 引用本文： | 张丽,石玮,张雷,李杰,李娜,安鹏丽,张悦,王蓓,孟明,王家鑫.负载灭活口蹄疫病毒树突状细胞启动的CD4~+T细胞应答[J].中国兽医科学,2011(7). |
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| 作者姓名： | 张丽 石玮 张雷 李杰 李娜 安鹏丽 张悦 王蓓 孟明 王家鑫 |
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| 作者单位： | 河北农业大学动物科技学院;河北大学医学部; |
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| 基金项目： | 国家自然科学基金项目(30972168) |
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| 摘 要： | 为研究负载灭活口蹄疫病毒(iFMDV)树突状细胞对CD4+T细胞的活化效应,用重组粒细胞-巨噬细胞集落刺激因子(rmGM-CSF)和白介素-4(rmIL-4)将小鼠外周血单核细胞诱导分化成树突状细胞(MoDCs),以信号阻断法制备淋巴结CD4+T细胞,利用负载iFMDV的MoDCs与CD4+T细胞共培养,以iFMDV+MoDCs作为对照,用ELISA检测不同时间培养上清液中IFN-γ的含量。用溶酶体抑制剂处理MoDCs,2h后再负载iFMDV,并与CD4+T细胞共培养,对照组则用iFMDV+MoDCs+CD4+T细胞,用ELISA检测不同时间培养上清液中IFN-γ的含量。结果显示,在共培养后第9和48小时,CD4+T细胞组与对照组比较差异均极显著(P<0.01)。共培养后第24和36小时,CD4+T细胞组与对照组比较均有显著性差异(P<0.05)。溶酶体抑制剂处理后,共培养第9、24、36和48小时组与对照组比较,均有显著性差异(P<0.05)。这表明MoDCs可通过溶酶体-MHC-Ⅱ途径向CD4+T细胞提呈iFMDV抗原,并且活化的CD4+T细胞可分化成Th1细胞。
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| 关 键 词： | 口蹄疫病毒 树突状细胞 CD4 T细胞 γ干扰素 |
CD4~+ T lymphocyte response triggered by dendritic cells pulsed with inactivated foot-and-mouth disease virus |
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| ZHANG Li,SHI Wei,ZHANG Lei,LI Jie,LI Na,AN Peng-li,ZHANG Yue,WANG Bei,MENG Ming,WANG Jia-xin.CD4~+ T lymphocyte response triggered by dendritic cells pulsed with inactivated foot-and-mouth disease virus[J].Veterinary Science in China,2011(7). |
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| Authors: | ZHANG Li SHI Wei ZHANG Lei LI Jie LI Na AN Peng-li ZHANG Yue WANG Bei MENG Ming WANG Jia-xin |
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| Institution: | ZHANG Li1,SHI Wei1,ZHANG Lei1,LI Jie1,LI Na1,AN Peng-li1,ZHANG Yue1,WANG Bei2,MENG Ming1,WANG Jia-xin1(1.College of Animal Science and Technology,Agricultural University of Hebei,Baoding 071000,China,2.Health Science Center,Hebei University,China) |
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| Abstract: | To investigate the activation of CD4+ T cells by dendritic cells pulsed with inactivated foot-and-mouth disease virus(iFMDV) in vitro,monocyte-derived dendritic cells(MoDCs) were generated by culturing murine peripheral blood monocytes with addition of rmGM-CSF and rmIL-4 in the medium.CD4+ T cells were harvested by blocking of CD8+T cells with anti-CD8 antibody in the lymph node T cell pool.MoDCs pulsed with iFMDV were co-cultured with CD4+ T cells,while iFMDV pulsed-MoDCs used as control.The release of th... |
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