| 小反刍兽疫病毒H和F蛋白多抗原表位的表达及其反应原性 |
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| 引用本文： | 石晓妮,窦永喜,张海瑞,翟军军,蒙学莲,曾巧英,才学鹏.小反刍兽疫病毒H和F蛋白多抗原表位的表达及其反应原性[J].中国兽医科学,2011(5). |
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| 作者姓名： | 石晓妮 窦永喜 张海瑞 翟军军 蒙学莲 曾巧英 才学鹏 |
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| 作者单位： | 甘肃农业大学动物医学院;中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室; |
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| 基金项目： | 国家公益性行业(农业)科研专项经费项目(200803018); 国家现代农业产业技术体系建设专项(nycytx-40-3) |
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| 摘 要： | 将通过计算机软件预测并通过间接ELISA检测确定的小反刍兽疫病毒(PPRV)H、F蛋白的10个B细胞抗原表位串联,再加上一个外源通用T细胞表位,表位之间用甘氨酸间隔,人工合成一条多抗原表位编码基因序列,将其插入原核表达载体pET30a(+),转化BL21(DE3)诱导表达。结果获得分子质量为19 ku的表达蛋白,该蛋白可与PPRV感染阳性血清发生特异性免疫反应。结果表明人工合成的PPRV多抗原表位编码基因可以在原核表达系统中表达,表达产物具有反应原性,这为研究PPRV抗原表位疫苗奠定了基础。
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| 关 键 词： | 小反刍兽疫病毒 重组多抗原表位 原核表达 间接ELISA |
Expression of multi-epitopes of H and F protein of peste des petits ruminants virus and analysis of their reactogenicity |
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| SHI Xiao-ni,DOU Yong-xi,ZHANG Hai-rui,ZHAI Jun-jun,MENG Xue-lian,ZENG Qiao-ying,CAI Xue-peng.Expression of multi-epitopes of H and F protein of peste des petits ruminants virus and analysis of their reactogenicity[J].Veterinary Science in China,2011(5). |
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| Authors: | SHI Xiao-ni DOU Yong-xi ZHANG Hai-rui ZHAI Jun-jun MENG Xue-lian ZENG Qiao-ying CAI Xue-peng |
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| Institution: | SHI Xiao-ni1,2,DOU Yong-xi2,ZHANG Hai-rui2,ZHAI Jun-jun2,MENG Xue-lian2,ZENG Qiao-ying1,CAI Xue-peng2(1.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China,2.State Key Laboratory of Veterinary Etiological Biology/Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China) |
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| Abstract: | Ten B cell antigen epitopes were predicted according to the sequence of H and F protein genes of peste des retits ruminants virus(PPRV) by the computer software,and then selected by indirect-ELISA.The ten epitopes and an universal helper T lymphocyte epitope were synthesized one by one,these epitopes are compared by Glycin to keep independent,the recombinant multi-epitope antigen gene was inserted into the prokaryotic expression vector pET-30a(+),the recombinant pET-30(a) was transformed into Escherichia co... |
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| Keywords: | peste des petits ruminants virus recombinant multi-epitope prokaryotic expression indirect-ELISA |
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