| 广东首株犬源贾第虫的基因型鉴定 |
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| 引用本文： | 朱海波,李国清,张萍,李结,李金平,蔺智兵.广东首株犬源贾第虫的基因型鉴定[J].中国兽医科学,2011(2). |
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| 作者姓名： | 朱海波 李国清 张萍 李结 李金平 蔺智兵 |
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| 作者单位： | 华南农业大学兽医学院; |
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| 基金项目： | 国家自然科学基金资助项目(30972179) |
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| 摘 要： | 为了对广东首株犬源贾第虫进行分离鉴定,采用常规方法分离犬粪便中的贾第虫包囊,通过显微镜观察对其进行形态学鉴定,并以小亚基核糖体RNA(16SrRNA)基因和谷氨酸脱氢酶(gdh)基因作为遗传标记,采用PCR方法对其进行了扩增、克隆、序列测定和分析。将扩增序列与GenBank中登录的参考序列进行比对,并用DNAStar软件建立系统进化树,以确定该贾第虫的基因型。结果显示,显微镜观察的结果与所报道的蓝氏贾第虫的形态一致;PCR扩增产物经10g/L琼脂糖凝胶电泳检测,出现299和432bp的片段,与预期结果一致;16SrRNA基因的序列分析结果显示该犬源贾第虫的基因型为A型,对gdh基因序列的进一步分析显示该基因型为A1亚型。结果表明本次分离的犬源贾第虫属于蓝氏贾第虫人兽共患的基因型。关键词:犬;蓝氏贾第虫;基因型
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| 关 键 词： | 犬 蓝氏贾第虫 基因型 |
Genotype identification of Giardiafrom dogs in Guangdong |
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| ZHU Hai-bo,LI Guo-qing,ZHANG Ping,LI Jie,LI Jin-ping,LIN Zhi-bing.Genotype identification of Giardiafrom dogs in Guangdong[J].Veterinary Science in China,2011(2). |
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| Authors: | ZHU Hai-bo LI Guo-qing ZHANG Ping LI Jie LI Jin-ping LIN Zhi-bing |
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| Institution: | ZHU Hai-bo,LI Guo-qing,ZHANG Ping,LI Jie,LI Jin-ping,LIN Zhi-bing(College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China) |
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| Abstract: | To identify the genotype of Giardia strain isolated the first time from dogs in Guangdong,the Giardia cysts were isolated from fecal material by the traditional method,the morphologic identification was based on microscopic observation,and the amplification,cloning and sequence analysis of their 16 S rRNA gene and gdh gene as genetic marker were carried out by PCR.The amplicon sequences were aligned with reference sequences in GenBank,and the phylogenetic trees were established by DNAStar software for their... |
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| Keywords: | dog Giardia lamblia genotype |
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