| 莱姆病间接ELISA诊断方法的建立及应用 |
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| 引用本文： | 杨吉飞,关贵全,牛庆丽,刘志杰,李有全,刘军龙,马米玲,任巧云,刘爱红,罗建勋,殷宏.莱姆病间接ELISA诊断方法的建立及应用[J].中国兽医科学,2011(7). |
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| 作者姓名： | 杨吉飞 关贵全 牛庆丽 刘志杰 李有全 刘军龙 马米玲 任巧云 刘爱红 罗建勋 殷宏 |
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| 作者单位： | 中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃省动物寄生虫病重点实验室农业部草食动物疫病重点实验室; |
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| 基金项目： | 国家引进国际先进农业科学技术计划(948)项目(2010-S04); 国家重点基础研究发展计划(973)项目(2010CB530206); 国家自然科学基金资助项目(30800820,30972182,31072130,31001061); 甘肃省重点项目(1002NKD035,0801NKD033); 农业部肉牛牦牛产业体系项目(CARS-38); 欧盟ASFRISK(211691); EPI-ZONE(FOOD-CT-2006-016236); ARBOZOONET(211757); PIROVAC(KBBE-3-245145) |
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| 摘 要： | 使用培养的狭义伯氏疏螺旋体B31菌株制备可溶性抗原,建立羊莱姆病血清抗体的间接ELISA诊断方法。应用伯氏疏螺旋体阴性、阳性血清评价该方法的特异性和敏感性。根据所建立的方法对从甘肃省甘南地区采集的450份羊血清进行检测。结果显示,该方法对羊莱姆病的阳性检出率和阴性符合率分别为90.1%和90.0%,与羊霉形体、布鲁氏菌、弓形虫以及羊无浆体、巴贝斯虫、泰勒虫等常见蜱传播病原的阳性血清之间没有交叉反应。甘肃省甘南地区夏河县、临潭县和卓尼县羊莱姆病的感染率分别为3.3%、6.1%和4.1%,甘南地区的平均感染率为5.1%。结果表明,本研究所建立的方法能够用于羊莱姆病的血清学诊断,甘南地区可能存在莱姆病的自然疫源地。
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| 关 键 词： | 莱姆病 伯氏疏螺旋体 可溶性抗原 间接酶联免疫吸附试验 |
Development and application of an indirect ELISA for diagnosis of Lyme disease |
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| YANG Ji-fei,GUAN Gui-quan,NIU Qing-li,LIU Zhi-jie,LI You-quan,LIU Jun-long,MA Mi-ling,REN Qiao-yun,LIU Ai-hong,LUO Jian-xun,YIN Hong.Development and application of an indirect ELISA for diagnosis of Lyme disease[J].Veterinary Science in China,2011(7). |
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| Authors: | YANG Ji-fei GUAN Gui-quan NIU Qing-li LIU Zhi-jie LI You-quan LIU Jun-long MA Mi-ling REN Qiao-yun LIU Ai-hong LUO Jian-xun YIN Hong |
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| Institution: | YANG Ji-fei,GUAN Gui-quan,NIU Qing-li,LIU Zhi-jie,LI You-quan,LIU Jun-long,MA Mi-ling,REN Qiao-yun,LIU Ai-hong,LUO Jian-xun,YIN Hong(State Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Veterinary Parasitology of Gansu Province/Key Laboratory of Grazing Animal Diseases of the Ministry of Agriculture/Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China) |
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| Abstract: | An indirect ELISA for diagnosis of Lyme disease in sheep was developed with soluble antigen prepared from Borrelia burgdorferi sensu stricto B31 strain.Specificity and sensitivity of the ELISA were evaluated by the known positive and negative sera.A total of 450 sera from sheep in Gannan Zangzu Autonomous Region of Gansu Province were examined by the ELISA.Positive coincidence of the known positive sera from sheep was 90.1% and negative coincidence of the known negative sera was 90.0% in the indirect ELISA.... |
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| Keywords: | Lyme disease Borrelia burgdorferi sensu lato soluble antigen indirect ELISA |
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