| 鸡马立克氏病病毒HC135分离株的致病性 |
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| 引用本文： | 张艳萍,刘长军.鸡马立克氏病病毒HC135分离株的致病性[J].中国兽医科学,2011(3). |
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| 作者姓名： | 张艳萍 刘长军 |
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| 作者单位： | 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室禽传染病研究室; |
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| 基金项目： | 国家重点实验室基本科研业务费专项(SKLVBP201008); 中央级公益性科研院所基本科研业务费专项(1610302011002) |
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| 摘 要： | 从辽宁省某鸡场疑似马立克氏病发病鸡外周血淋巴细胞中分离到1株适应鸭胚成纤维细胞生长的马立克氏病病毒(MDV),经蚀斑克隆和细胞传代,成功培育了1株增殖性能稳定的MDV细胞毒株,命名为HC135株;将HC135株第4代细胞培养物分别人工感染非免疫和不同疫苗免疫的SPF鸡,分析其对鸡的致病力,分离毒株攻毒对照组鸡的发病率为100%(10/10),死亡率为30%(3/10);HVT疫苗及HVT/SB1双价疫苗对分离毒株的保护指数分别为22.2和40.0。结果表明,该毒株可以突破HVT疫苗和HVT/SB1双价疫苗的免疫保护,具有特超强毒株(vv+MDV)的特点;也证实了我国商品鸡群中存在vv+MDV毒株。同时比较了HC135株与14株参考毒株的Meq基因序列,发现其Meq基因的ORF内没有基因片段的插入或缺失,推导的氨基酸存在点突变,这种突变符合高毒力毒株的变异规律。
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| 关 键 词： | 马立克氏病病毒 致病性 Meq基因 分离毒株 |
Pathogenicity of Marek's disease virus field strain HC135 |
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| ZHANG Yan-ping,LIU Chang-jun.Pathogenicity of Marek's disease virus field strain HC135[J].Veterinary Science in China,2011(3). |
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| Authors: | ZHANG Yan-ping LIU Chang-jun |
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| Institution: | ZHANG Yan-ping,LIU Chang-jun(State Key Laboratory of Veterinary Biotechnology/Division of Avian Infectious Diseases,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China) |
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| Abstract: | Peripheral blood lymphocytes of the diseased chickens from commercial chicken flocks experienced significant Marek's disease visceral tumour in Liaoning Province were collected,and were inoculated into monolayer of duck embryo fibroblast.The isolated Marek's disease virus(MDV) was obtained by plaque clone,and named strain HC135.To examine the pathogenicity of this isolate,unvaccinated SPF chickens were challenged with strain HC135.Morbidity and mortality were 100%(10/10) and 30%(3/10),respectively.HVT vacci... |
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| Keywords: | Marek's disease virus pathogenicity Meq gene field virus strain |
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