| 五种中草药对弓形虫体外增殖的抑制效果 |
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| 引用本文： | 荆振宇,蒋蔚,王权,陈永军,杨康,宋宁宁,崔常勇.五种中草药对弓形虫体外增殖的抑制效果[J].中国兽医科学,2011(2). |
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| 作者姓名： | 荆振宇 蒋蔚 王权 陈永军 杨康 宋宁宁 崔常勇 |
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| 作者单位： | 中国农业科学院上海兽医研究所农业部动物寄生虫学重点开放实验室;东营出入境检验检疫局; |
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| 基金项目： | 公益性行业(农业)科研专项(200803017) |
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| 摘 要： | 探索治疗弓形虫病的有效药物组方,从而为临床合理用药提供科学的指导,通过体外试验,培养Ana-1细胞作为弓形虫宿主细胞,采用MTT法测定了甘草、黄芩、百部、苦参和贯众5种中药对细胞的安全浓度,将弓形虫RH株速殖子与Ana-1细胞在含不同浓度药物的细胞培养液中共同培养,在加药后第4、8、12、24、48小时,观察细胞和弓形虫的生长状态和形态,选取第48小时观察药物体外抑制虫体增殖的效果,并以磺胺嘧啶钠作为阳性药物对照。结果显示,黄芩、百部、甘草可显著抑制弓形虫的增殖,而贯众和苦参效果不佳。
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| 关 键 词： | 刚地弓形虫 中草药 体外 抑制 |
Inhibition effects of five Chinese herbal medicines on Toxoplasma gondii proliferation in vitro |
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| JING Zhen-yu,JIANG Wei,WANG Quan,CHEN Yong-jun,YANG Kang,SONG Ning-ning,CUI Chang-yong.Inhibition effects of five Chinese herbal medicines on Toxoplasma gondii proliferation in vitro[J].Veterinary Science in China,2011(2). |
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| Authors: | JING Zhen-yu JIANG Wei WANG Quan CHEN Yong-jun YANG Kang SONG Ning-ning CUI Chang-yong |
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| Institution: | JING Zhen-yu1,JIANG Wei1,WANG Quan1,CHEN Yong-jun1,YANG Kang1,SONG Ning-ning1,CUI Chang-yong2(1.Key Laboratory of Veterinary Parasitology of the Ministry of Agriculture/Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200241,China,2.Dongying Entry-Exit Inspection and Quarantine Bureau,Dongying 257091,China) |
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| Abstract: | To study the effective treatment of Chinese herbal medicine against toxoplasmosis and to provide scientific guidance for clinical use of drugs,the inhibiting effects of five Chinese herbal medicines such as Radix Glycyrrhizae,Radix Scutellariae,Radix Stemonae,Rhizoma Cyrtomii and Radix Sophorae flavescentis were observed on Toxoplasma gondii in vitro.tachyzoite of T.gondii RH strain was used to infect the Ana-1 cells in vitro,the safe concentrations of drugs on cell were determined by MTT assay,and then add... |
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| Keywords: | Toxoplasma gondii Chinese herbal medicine in vitro inhibition |
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