| 猪瘟病毒浙江分离株体外生长特性及其囊膜糖蛋白E2分子的变异分析 |
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| 引用本文： | 万婧,陈宁,童超,廖迅,谢文绮,李肖梁,方维焕.猪瘟病毒浙江分离株体外生长特性及其囊膜糖蛋白E2分子的变异分析[J].中国兽医科学,2011(8). |
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| 作者姓名： | 万婧 陈宁 童超 廖迅 谢文绮 李肖梁 方维焕 |
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| 作者单位： | 浙江大学浙江省动物预防医学重点实验室; |
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| 基金项目： | 浙江省教育厅2009年度科研计划项目(Y200909144) |
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| 摘 要： | 为了研究分离于浙江省衢州和杭州的2株猪瘟病毒基因2型流行毒株QZ-07、HZ1-08在猪肾细胞(PK-15)和猪睾丸细胞(ST)中的生长特性及其囊膜糖蛋白E2的分子进化特征,采用间接免疫荧光、效价测定和进化分析等分子生物学方法将基因2型流行毒株与基因1型石门株进行了比较。结果表明,石门株在PK-15细胞中的增殖效率明显高于ST细胞;感染PK-15细胞后第48小时,细胞内的效价可达107TCID50/mL,而在ST细胞中的效价只有105.25 TCID50/mL。基因2型毒株在PK-15细胞中的增殖水平明显低于ST细胞,尤其以HZ1-08株更为明显;感染ST细胞后第48小时,细胞内的效价为105 TCID50/mL,而PK-15细胞中的效价只有102.75 TCID50/mL。两基因型毒株E2的核苷酸同源性低于90%,氨基酸同源性约为90%。表明,基因2型毒株的体外生长特性及其E2的分子特性均与基因1型毒株存在差异。
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| 关 键 词： | 猪瘟病毒 生长特性 囊膜糖蛋白E2 分子变异 |
Growth characteristics of classical swine fever virus strains isolated in Zhejiang and genetic variations of their envelope glycoprotein E2 |
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| WAN Jing,CHEN Ning,TONG Chao,LIAO Xun,XIE Wen-qi,LI Xiao-liang,FANG Wei-huan.Growth characteristics of classical swine fever virus strains isolated in Zhejiang and genetic variations of their envelope glycoprotein E2[J].Veterinary Science in China,2011(8). |
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| Authors: | WAN Jing CHEN Ning TONG Chao LIAO Xun XIE Wen-qi LI Xiao-liang FANG Wei-huan |
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| Institution: | WAN Jing,CHEN Ning,TONG Chao,LIAO Xun,XIE Wen-qi,LI Xiao-liang,FANG Wei-huan(Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine,Zhejiang University,Hangzhou 310058,China) |
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| Abstract: | Two field strains QZ-07 and HZ1-08 of classical swine fever virus isolated from diseased pigs in Zhejiang were phylogeneticly classified as group 2. Their replication kinetics in porcine kidney 15(PK-15) and swine testicle(ST) cell lines were compared with the group 1 Shimen strain.The Shimen strain replicated more efficiently in PK-15 cells than in ST cells(107 TCID50/mL vs 105.25 TCID50/mL at 48 h post-infection).In contrast,the two field strains displayed decreased replication in PK-15 cells(102.75 TCID5... |
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| Keywords: | classical swine fever virus growth characteristics glycoprotein E2 genetic diversity |
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