大鼠死后肌肉总腺苷酸及腺苷酸能荷的时序性变化

目的测定大鼠死后不同时间双后肢肌肉嘌呤腺苷酸三磷酸腺苷(adenosine triphosphate,ATP)、二磷酸腺苷(adenosine diphosphate,ADP)、一磷酸腺苷(adenosine monophosphate,AMP)]含量,计算总腺苷酸(total adenine nucleotide,TAN)含量和腺苷酸能荷(adenylic-acid energy charge,AEC)的变化值并探究其与死亡时间(postmortem interval,PMI)的关系。方法健康大鼠颈部脱臼处死后,20℃恒温保存,分别于死后0、24、48、72、96、120、144、168 h提取大鼠双后肢肌肉,利用反相高效液相色谱法测定嘌呤腺苷酸含量,计算双后肢肌肉组织的TAN和AEC,并分别建立其与PMI关系的相关回归方程。结果大鼠死亡168h内,ATP、ADP变化趋势不同,AMP的含量持续增加;TAN值随PMI的延长而逐渐增加,而AEC在死后168h内,整体呈现下降趋势。AEC随PMI变化规律明显,决定系数较高(R2为0.903),曲线拟合关系较好;ATP、ADP、AMP、TAN与PMI拟合关系不佳(R2为0.198~0.754)。结论AEC的死后变化规律为法医学PMI推断提供了新的研究思路。

Sequential Changes of Total Adenine Nucleotide and Adenylic-Acid Energy Charge in Muscles of Rats after Death

Objective To determine the purine adenylateadenosine triphosphate(ATP),adenosine diphosphate(ADP),adenosine monophosphate(AMP)]content in the muscles of both hind limbs of rats at different postmortem interval(PMI),calculate the changes in the total adenine nucleotide(TAN)content and the adenylic-acid energy charge(AEC),and explore their relationship with PMI.Methods Healthy rats were sacrificed by cervical dislocation and kept at 20℃.The muscles of their hind limbs were extracted at 0,24,48,72,96,120,144,and 168 h after death.Reversed-phase high performance liquid chromatography was used to determine the content of purine adenylates,the TAN and AEC of the muscles of the both hind limbs were calculated,and the related regression equations of their relationship with PMI were established.Results Within 168 h of death of rats,the trend of ATP change was different from ADP,and the content of AMP continuously increased.The TAN value gradually increased with the extension of PMI,and the AEC showed a downward trend within 168 h after death.Among them,the patterns of AEC changes with PMI were obvious,the correlation coefficient was high(R2=0.903),and the curve fitting relationship was good;the fitting relationship between ATP,ADP,AMP,TAN and PMI was poor(R2=0.198-0.754).Conclusion The postmortem change patterns of AEC provide new research ideas for PMI estimation in the forensic field.