| 福尔马林固定石蜡包埋组织中DNA提取 |
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| 作者姓名： | Tan ZY Ding M |
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| 作者单位： | 中国医科大学法医学院法医血清教研室,辽宁,沈阳,110001 |
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| 摘 要： | 由于甲醛介导的DNA损伤和石蜡对DNA提取的阻碍作用,使得常规DNA提取方法很难从福尔马林固定石蜡包埋组织(FFPET)中获取高质量的DNA。近年来,众多学者的研究表明,通过改良预处理方法,优化蛋白酶K消化作用,简化DNA提取步骤,纯化DNA提取物等,可以有效提高FFPET中提取的DNA质量,为FFPET的DNA分析奠定了基础。
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| 关 键 词： | 福尔马林固定 石蜡包埋组织 DNA提取 |
| 文章编号： | 1004-5619(2006)06-0455-04 |
| 修稿时间： | 2006年4月12日 |
DNA extraction from formalin fixed and paraffin embedded tissues |
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| Tan ZY,Ding M.DNA extraction from formalin fixed and paraffin embedded tissues[J].Journal of Forensic Medicine,2006,22(6):455-458. |
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| Authors: | Tan Zhuo-yi Ding Mei |
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| Institution: | School of Forensic Medicine, China Medical University, Shenyang 110001, China. kukler@sina.com |
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| Abstract: | Owing to the DNA degradation induced by formalin and the obstruction of paraffin to DNA extraction, it is difficult to recover high-quality DNA from Formalin fixed and paraffin embedded tissue(FFPET).In recent years, a lot of researches indicate that the DNA extraction from FFPET can be developed by improving the pretreatment,optimizing the digestion condition of proteinase, simplizing the procedures of the DNA extraction, purifying the extracted DNA and so on, which may pave a way for popularizing FFPET in DNA analysis. |
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| Keywords: | formalin fixed paraffin embedded tissue DNA extraction |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ |
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