| 抗脂肪细胞特异膜蛋白单链抗体基因的克隆及序列分析 |
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| 引用本文： | 汪磊,高士争,张曦,郝军元,张玉静.抗脂肪细胞特异膜蛋白单链抗体基因的克隆及序列分析[J].中国兽医科学,2004,34(2):11-16. |
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| 作者姓名： | 汪磊 高士争 张曦 郝军元 张玉静 |
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| 作者单位： | 1. 云南农业大学,云南省动物营养与饲料重点实验室,云南,昆明,650201
2. 中国人民解放军军需大学,生化教研室,吉林,长春,130062 |
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| 基金项目： | 国家自然科学基金项目 (30 2 6 0 0 79) |
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| 摘 要： | 从分泌抗 4 0ku脂肪细胞特异膜蛋白单克隆抗体的杂交瘤细胞中提取纯化mRNA ,经RT PCR扩增抗体轻、重链可变区基因 ,然后与Linker连接组装成单链抗体基因 (scFv)。结果 ,成功地构建了scFv基因 ,其排列方式为VH linker VL。经序列分析表明 ,scFv基因全长 74 1bp ,VH基因全长 36 9bp ,VL基因全长 32 7bp。抗脂肪细胞膜蛋白单链抗体基因的构建为进行该抗体基因的表达奠定了基础
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| 关 键 词： | 脂肪细胞膜蛋白 单链抗体 序列 |
| 文章编号： | 1000-6419(2004)02-0011-06 |
| 修稿时间： | 2003年9月28日 |
Cloning and sequence analysis of scFv gene |
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| WANG Lei.Cloning and sequence analysis of scFv gene[J].Veterinary Science in China,2004,34(2):11-16. |
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| Authors: | WANG Lei |
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| Abstract: | With the purified mRNA from hybridoma which secrete monoclonal antibodies against a 40 ku adipocyte plasma membrane protein, the V_(H) (heavy-chain variable region) and V_(L) (light-chain variable region) genes encoding the monoclonal antibody were amplified by RT-PCR, then they were joined with Linker for construction of scFv gene. The results showed that the scFv gene had been constructed in the way of V_(H)-linker-V_(L). Sequence analysis demonstrated that length of the scFv gene was 741 bp and length of V_(H) and V_(L) genes was 369 bp and 327 bp respectively. The constructed scFv gene can be used to express the antibody. |
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| Keywords: | adipocyte plasma membrane scFv sequence |
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