外伤后大脑癫痫灶中SLIT-2的表达变化

目的通过测定人体大脑外伤后癫痫灶中轴突再生调控因子SLIT-2的表达情况,探讨其在外伤后癫痫鉴定中的应用意义。方法 75例人大脑皮质样本分为3组:①实验组:为脑外伤后癫痫患者(30例),②对照组1:为非外伤性癫痫患者(30例),③对照组2:为车祸致颅脑损伤死亡者(15例)。实验组和对照组1样本来源于癫痫手术所切除的脑皮质(患者或其近亲属知情,自愿提供);对照组2样本来源于尸体解剖的大脑皮质。通过HE染色、免疫组化染色、westernblot和图像分析技术,观察、检测上述样本SLIT-2的表达情况,所得数据经统计学处理分析。结果 SLIT-2在实验组与对照组1癫痫灶皮质的神经细胞膜上呈阳性表达,在对照组2中仅有微量表达。实验组SLIT-2的表达高于对照组1,两组间的差异具有显著性意义(P0.05);显著高于对照组2,两组间的差异具有显著性意义(P0.05)。结论 SLIT-2有望作为区别外伤后癫痫与非外伤性癫痫的病理指标之一,通过进一步研究,逐步运用到法医学的鉴定实践之中,为外伤后癫痫的法医病理学鉴定提供客观依据。

The expression of SLIT-2 in the epileptic cortex in the cases of post traumatic epilepsy

Objective To investigate the value of SLIT-2 expression in the epileptic cortex in the patient with post traumatic epilepsy.Methods All samples from 75 cases were divided into 3 groups.1.Post traumatic epilepsy(PTE,30 cases): The specimen cut from the human epileptic cortex(mainly in temporal) during operation of patient with post traumatic epilepsy.2.Control group 1(epilepsy without injury,30 cases): The specimen removed from the human epileptic cortex(mainly in temporal) during operation of non-post-traumatic epilepsy.3.Control group 2(15 autopsied cases): The specimen cut from the cerebral cortex of corpse died from hemorrhagic shock.The expression of SLIT-2 in the cortex was observed and detected by immunohistochemistry,western blot electrophoresis and image analytic techniques and the data of SLIT-2 expression was analyzed and compared statistically among three groups.Results There was positive staining of SLIT-2 on the neuron membrane in the group of the PTE and slight positive staining in the control group 1,but negative staining in the control group 2.The quantity of SLIT-2 in the PTE groups was greater than that of control group 1(P<0.05) and was significantly greater than that of control group 2(P<0.05).Conclusion SLIT-2 can be used as one of targets to distinguish the PTE and non-PTE and it may be gradually used in the practice of medicolegal expertise to offer the objective evidence.