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| 低拷贝DNA模板检验方法探讨 | |
| 引用本文： | 袁丽,鲁涤,刘耀.低拷贝DNA模板检验方法探讨[J].中国法医学杂志,2009,24(6). |
| 作者姓名： | 袁丽 鲁涤 刘耀 |
| 作者单位： | 1. 中国政法大学证据科学教育部重点实验室,北京100088;中国人民公安大学,北京100038 2. 中国政法大学证据科学教育部重点实验室,北京,100088 3. 公安部物证鉴定中心,北京,100038 |
| 基金项目： | 北京市教育委员会共建项目建设计划资助 |
| 摘 要： | 目的探讨扩增及检测方法对低拷贝DNA模板分型检测灵敏度的影响。方法Control DNA 9947A按比例稀释,采用IdentifilerTM和DNATyper15TM试剂扩增,循环参数设置为28和28+6个循环,平行扩增3次,分别单独检测及3次合并检测,使用310及3130分析仪检测。结果28+6个循环的基因座检出率高于28个循环;等位基因不平衡及丢失与基因座没有特异性的关联,随着DNA模板量的减少,等位基因不平衡及丢失增多;将3次扩增产物混合后检测,等位基因不平衡及丢失情况减少,分型正确率增高。结论模板DNA分3次扩增后混合检测、循环数为28+6,可提高低拷贝模板基因座的检出率。 |
| 关 键 词： | 法医物证学 低拷贝模板 短串联重复序列 基因分型 |
Study on the amplifying and typing of low copy number DNA |
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| YUAN Li,LU Di,UU Yao.Study on the amplifying and typing of low copy number DNA[J].Chinese Journal of Forensic Medicine,2009,24(6). | |
| Authors: | YUAN Li LU Di UU Yao |
| Institution: | YUAN Li1,2,LU Di1,LIU Yao3 / 1.China University of Political Science and Law's Key Laboratory of Evidence Science,Ministry of Education,Beijing 100088,China,2.Chinese People's Public Security University,Beijing 100038,3.Institute of Forensic Science,Ministry of Public Security |
| Abstract: | Objective To study the influence on STR typing for low copy number(LCN) DNA using different methods of amplification and detection.Methods Control DNA 9947A was diluted and then amplified with IdentifilerTM or DNATyper15TM.The heat cycles were set to 28 or 28 add 6.Each template was amplified three times in parallel,and then the amplified products or the product mixture of three amplifictions were deteced with 310 or 3130 Genetic Analyzer.Results The success rate of STR typing with the method 28+6 cycles wa... |
| Keywords: | forensic biological evidence low copy number DNA short tandem repeats genotyping |
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