中国汉族及日本群体7个Y—STR位点及单倍型的遗传多态性和群体差异

目的调查Y染色体7个STR位点及单倍型的遗传多态性并分析其群体差别。方法应用PCR、变性聚丙烯酰胺凝胶电泳结合银染显色分型技术,检测45例中国汉族及59例日本男性DNA样品。结果在DYS393、DYS389Ⅰ、DYS19、DYS390、DYS389Ⅱ、DYS392等6个位点中共检出33个等位基因,DYS385位点检出39个等位基因组,其频率分布在0.0169～0.6444之间,DP值分布在0.5406～0.9579之间,以DYS385位点最高。7个位点数据综合比较,二组群体间在遗传学上存在显著性差异P<0.05。由7个位点组成的单倍型有95种,中国汉族有41种,DP值为0.9960,日本群体有54种,DP值为0.9965,2群体间未发现相同的单倍型。结论上述7个STR位点属于高鉴别能力位点,单倍型具有很高的遗传多态性并显示出明显的民族特征。

Genetic polymorphism and the population difference of 7 Y-STR loci and haplotypes in Chinese Han and Japanese populations

OBJECTIVE: To investigate the genetic polymorphism of 7 Y-STR loci and haplotypes and analyze their population difference. METHODS: The DNA samples from 45 Chinese Han and 59 Japanese men were analyzed by PCR and the denatured PAGE followed by silver staining. RESULTS: 33 alleles were identified for 6 loci including DYS393, DYS389 I, DYS19, DYS390, DYS389 II and DYS392, 39 alleles were identified for DYS 385, the frequency distributions were between 0.0169-0.6444, the DP value distributions of them were between 0.5406-0.9579, the DP value of DYS 385 was the highest. With the comparison of data of the 7 loci, significant difference in genetics was observed between the two populations(P < 0.05). There were 95 haplotypes that consisted of the 7 loci, Chinese Han had 41 haplotypes, DP value 0.9960, Japanese population had 54 haplotypes, DP value 0.9965, no common haplotypes were found between them. CONCLUSION: The 7 STR loci above belong to loci of high discriminating ability, the haplotypes are highly polymorphic and their ethical character is apparent.