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| 电击死兔骨骼肌及心肌HSP70 mRNA和c-fos mRNA表达 | |
| 作者姓名： | Wang Y Liu M Cheng WB He GQ Li F Liao ZG |
| 作者单位： | 1. 四川大学华西基础医学与法医学院,四川,成都,610041;四川大学,华西公共卫生学院,四川,成都,610041 2. 四川大学华西基础医学与法医学院,四川,成都,610041 3. 四川大学,华西公共卫生学院,四川,成都,610041 4. 四川大学华西基础医学与法医学院,四川,成都,610041;河南科技大学,法医学院,河南,洛阳,471003 |
| 摘 要： | 目的 研究电击死兔骨骼肌与心肌HSP70 mRNA和c-fos mRNA-表达变化。探究生前电击与死后电击的鉴别方法。方法 15只新西兰兔,随机分电击死组、死后电击组和对照组,每组5只,用荧光RT-PCR技术检测骨骼肌与心肌热休克蛋白70（HSP70） mRNA与c-fos mRNA表达水平,对所得结果进行统计学分析。结果 生前电击兔骨骼肌及心肌HSP70 mRNA与c-fos mRNA表达高于死后即刻电击者（P〈0．05）。结论 检测骨骼肌及心肌HSP70 mRNA与c-fos mRNA表达变化有助于于生前电击与死后电击的鉴别。 |
| 关 键 词： | 法医病理学 电击伤 荧光光度测定法 HSP 70热休克蛋白质类 基因 fos 逆转录聚合酶链反应 |
The expressions of HSP 70 mRNA and c-fos mRNA in the skeletal muscle and cardiac muscle of rabbits by electrocuted |
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| Wang Y,Liu M,Cheng WB,He GQ,Li F,Liao ZG.The expressions of HSP 70 mRNA and c-fos mRNA in the skeletal muscle and cardiac muscle of rabbits by electrocuted[J].Journal of Forensic Medicine,2008,24(4):245-247,251. | |
| Authors: | Wang Ye Liu Min Cheng Wei-bo He Gui-qiong Li Fan Liao Zhi-gang |
| Institution: | West China school of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China. yellpath007@scu.edu.cn |
| Abstract: | Objective To study the changes of HSP 70 mRNA and c-fos mRNA expression and to find a method to differentiate antemortem from postmortem electrocution.Methods Fifteen New Zealand rabbits were randomly divided into three groups,the antemortem electrocution group,the postmortem electrocution group,and the control group.Each group consists of five rabbits.The levels of HSP 70 mRNA and c-fos mRNA in skeletal muscle and cardiac muscle were examined with quantitative fluorescent RT-PCR.Results The levels of HSP 70 mRNA and c-fos mRNA in the antemortem electrocution group increased significantly(P<0.05),compared with that of the postmortem electrocution group.Conclusion The changes of HSP 70 mRNA and c-fos mRNA expression in skeletal muscle and cardiac muscle can be used as an indicator to distinguish antemortem from postmortem electrocution. |
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| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ | |