| 应用等电聚焦电泳对人精液黄递酶Ⅲ表型分布的研究 |
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| 引用本文： | 杨庆恩,黄秋菊,杨荣芝,梅棍. 应用等电聚焦电泳对人精液黄递酶Ⅲ表型分布的研究[J]. 中国法医学杂志, 1989, 0(3) |
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| 作者姓名： | 杨庆恩 黄秋菊 杨荣芝 梅棍 |
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| 作者单位： | 同济医科大学法医学系，同济医科大学法医学系，同济医科大学法医学系，同济医科大学法医学系 |
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| 摘 要： | 应用超薄层聚丙烯酰胺凝胶等电聚焦电泳对武汉地区198例成人精液黄递酶Ⅲ(DIA_3)作了分型。观察到4种表现型,基因频率分别为DIA_3~1=0.7727;DIA_3~2=0.2172;DIA_3~3=0.0101。对保存精斑进行检测证实,室温下保存9周的精斑仍能作DIA_3分型。在聚丙烯酰胺凝胶中引入分离剂HEPES,提高了分辨率。改进后的等电聚焦技术尤适于DIA_3基因产物的分离。文中还调查了我国汉族人群DIA_3频率分布,并与其它群体资料作了比较。
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| 关 键 词： | 黄递酶 等电聚焦 基因频率 精斑 分离剂 |
A STUDY OF THE PHENOTYPE DISTRIBUTION OF DIA_3 IN THE HUMAN SEMEN USING IEF |
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| Yang Qing''''en,Huang Qiuju,Yang Rongzhi,Mei Kun Facutty of Forensic medicine,Tong Ji Medical University,Wuhan. A STUDY OF THE PHENOTYPE DISTRIBUTION OF DIA_3 IN THE HUMAN SEMEN USING IEF[J]. Chinese Journal of Forensic Medicine, 1989, 0(3) |
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| Authors: | Yang Qing''''en Huang Qiuju Yang Rongzhi Mei Kun Facutty of Forensic medicine Tong Ji Medical University Wuhan |
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| Abstract: | Human semen DIA_3 phenotyping was carried out by isoelectric focusing on ultrathinlayer polyacry-lamide gel. The phenotype distribution of DIA_3 in human samples from 198 unrelated Chinese male wereobserved. The DIA_3 allele frequencies were calculated: DIA_3~1= O. 7727, DIA_3~2= O. 2172, DIA_3~3= O. 0101.In our experiments, the separator HEPES was applied to the polyacrylamide gel. The sensitivity phenotypingwas then incredsed. This method is suitable to detect the products of DIA_3~3 gene frequencies in the presentstudy were compared with those in other population. |
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| Keywords: | Diaphorase(DIA_3) Isoelectric focusing Gene frequency Seminal stain Separator |
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