| 陈旧骨骼DNA提取 |
| |
| 作者姓名： | Zhang J Xue QJ Tang GF Peng L |
| |
| 作者单位： | 1. 泰安市公安局刑警支队,山东,泰安,271000
2. 济宁医学院微生物教研室,山东,济宁,272400 |
| |
| 摘 要： | 目的寻找从陈旧骨骼中提取DNA的有效方法。方法运用传统的有机法结合Microcon100纯化柱提取骨骼DNA。结果用常规荧光标记复合STR基因分型法可对提取到的陈旧骨骼DNA进行成功分析。结论有机法结合Micrcon100纯化柱提取陈旧骨骼DNA法可有效应用于实际检案。
|
| 关 键 词： | 有机法 Microcon100 DNA提取 陈旧骨骼 |
| 文章编号： | 1004-5619(2006)01-0072-02 |
| 修稿时间： | 2005年4月11日 |
DNA extraction from old bones |
| |
| Zhang J,Xue QJ,Tang GF,Peng L.DNA extraction from old bones[J].Journal of Forensic Medicine,2006,22(1):72-73. |
| |
| Authors: | Zhang Jie Xue Qing-jie Tang Guang-feng Peng Lei |
| |
| Institution: | Criminal Police Department of Taian City Public Security Bureau, Taian 271000, China. zxingda@163.com |
| |
| Abstract: | Objective To explore an efficient method for extracting DNA from old bones. Methods Using an organic combined with and Microcon 100 to extract and purify DNA. Results The extracted DNA was successfully genotyped by using florescence labeling STR multiplex amplification. Conclusion This method will be useful for forensic scientists in identification of DNA from old bones. |
| |
| Keywords: | organic method microcon100 DNA extraction old bones |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ |
