直接进样气相色谱法测定全血中乙醇的含量

目的建立一种直接进样气相色谱法检测全血中的乙醇。方法全血经硫酸铝钾沉淀蛋白,加入内标异丙醇后,用直接进样气相色谱法进行检测,FID为检测器,用保留时间定性,内标标准曲线法定量。结果该方法线性范围为0.2-1.4mg/m L,相关系数R=0.999 3,总分析时间不超过5min,最低检测限为0.01mg/m L,相对标准偏差（RSD）〈5%,平均回收率为92.3%;所建立的方法与顶空气相色谱（HS-GC）法比较相对偏差（RD）〈10%。结论该方法可用于全血中乙醇的检测。

Determination of alcohol in whole blood by gas chromatography with direct injection

Objective To determine alcohol in whole blood,we established a method of gas chromatography with direct injection. Methods The protein of whole blood was firstly precipitated by using aluminum potassium sulfate,and then the internal standard substance（ such as isopropanol） was added to the whole blood. Finally,the whole blood was analyzed by gas chromatography with direct injection（ the detector was FID）. Retention time of a peak was used as qualitative analytical parameter,and an internal standard curve method was used as the quantitative analytical method. Results The curve was linear in the range from 0. 2mg / m L to 1. 4mg / m L,and its correlation coefficients（ R） came up to 0. 999 29. The total analytical time of the method was less than 5min,and the lowest limit of detection（ LLOD） was 0. 01 mg / m L in the method. The relative standard deviation（ RSD） and average recovery rate of the method were less than 5% and up to 92. 3%,respectively. The relative deviation（ RD） was less than 10% comparing the method＇s analytical result with the determining result of headspace gas chromatography（ HS-GC） method.Conclusion The method could be used to determine alcohol in whole blood.