Automation of high volume MPS mixture interpretation using CaseSolver

Sixty samples were sequenced using the ForenSeq kit to produce complex STR DNA mixtures, represented with three separate formats capturing different degrees of sequence information. All mixtures were run through the (open-source) CaseSolver software for comparison against both 10 reference profiles. By comparing the performance of the qualitative and the quantitative models for the different allele formats we found the following preliminary results: The quantitative model performed better than the qualitative model, however the gain was only substantial when the LR was already large. The performance gain of using the longest uninterrupted stretch over using only repeat units was large, whereas the further gain of also using the whole sequence information was small.