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Forensic examination and application of areca nuts as material evidence |
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| Authors: | Weitao Yan Yang Yu Yuxin Wang Shu Yang Xi Li Shuang Lu Lagabaiyila Zha Jifeng Cai |
| Institution: | 1. Department of Forensic Science, School of Basic Medical Science, Central South University, Changsha, Hunan, China;2. Department of Neurobiology and Human Anatomy, School of Basic Medical Science, Central South University, Changsha, Hunan, ChinaSearch for more papers by this authorYang Yu MBBS, Yuxin Wang BSc, Shu Yang MBBS, Xi Li MBBS, Shuang Lu MMed, Lagabaiyila Zha MD, Jifeng Cai MD,
First published: 04 October 2021
Funding information: ;3. This research was supported by the Key Program of National Natural Science Foundation of China (No. 82030058);4. the Project of Research Study and Innovative Experiment Plan for University Students in China's Hunan Province in 2018 (No.1091);5. the Fundamental Research Funds for the Central Universities of Central South University (No. 2020zzts218);6. and the Hunan Provincial Innovation Foundation For Postgraduate (No. CX20200116). |
| Abstract: | In this study, we aimed to explore the possibility of DNA analysis of areca nut as material evidence and the value of short tandem repeat (STR) typing of areca nut as material evidence under the condition of simulating external environment. In this study, water soaking, soil burial, sun exposure, and wet environment were used to treat areca nut residues. Chelex 100 was used to extract DNA, the PowerPlex21 kit to amplify, and the ABI PRISM® 310 Genetic Analyzer to analyze the DNA of areca nut residues. DNA and STR typing were performed to analyze the residue after chewing. The results showed that the number of residual sites decreased with time under the conditions of water soaking, soil burial, sun exposure, and wet environment. Thus, areca nut can be used as forensic material evidence for DNA analysis and individual identification. |
| Keywords: | areca nut capillary electrophoresis DNA genotyping DNA profiling forensic genetics individual identification STR typing |