广东壮族群体12个X-STR基因座的遗传多态性

目的 调查广东壮族群体DXS10103等12个 X-STR基因座的遗传多态性.方法 采用Investigator Argus X-12体系对200名广东壮族无关个体（男性100名,女性100名）进行12个X-STR的DNA分型.结果 该群体中12个X-STR基因座共检出143个等位基因,等位基因频率为0.0033～0.6433,等位基因分布均符合Hardy-Weinberg平衡.DXS10103与DXS10101基因座间存在连锁不平衡.各基因座的多态信息含量（PIC）为0.3944～0.9159,男性个体识别力（DPm）和女性个体识别力（DPf）分别为0.4815～0.9214和0.6441～0.9884,二联体和三联体的平均非父排除率分别为0.2625～0.8501（MECduo）和0.3944～0.9159（MECtrio）.累积男性个体识别力（CDPm）为0.999999998,累积女性个体识别力（CDPf）为0.999999999,累积二联体非父排除率（CMECduo）为0.999998271,累积三联体非父排除率（CMECtrio）为0.999999989.结论 Investigator Argus X-12系统在广东壮族群体中具有高度的多态性,本实验获得的群体数据可用于个体识别及亲缘关系鉴定案件的评估参考.

Genetic polymorphisms of 12 X-STR loci in Guangdong Zhuang population

Objective To evaluate the genetic polymorphism and forensic application of 12 X-STR loci in Zhuang Population of Guangdong Province. Methods Allele frequencies for 12 X-STR loci in 200 unrelated Zhuang individuals （100 males and 100 females） were determined and the genetic parameters for forensic application were calculated. Results No devitation of the ob served allele frequency from Hardy-Weinberg equilibrium expectations was found. DXS10103 and DXS10101 were found to be in linkage disequilibrium. 143 alleles were observed and allele frequencies varied from 0. 0033 to 0. 6433. The polymorphism information content （PIC） varied from 0. 3944 to 0. 9159. The power of discrimination for male and female （DPm and DPf） ranged from 0. 4815 to 0. 9214 and from 0. 6441 to 0. 9884, respectively. The mean exclusion chance for duo and trio cases ranged from 0. 2625 to 0. 8501 （MECduo） and from 0. 3944 to 0. 9159 （MECtrio）, respectively. The combined power of discrimination was 0. 999999998 in males （CDPm） and 0. 999999999 in females （CDPf）, and the combined mean exclusion chance was 0. 999998271 in duo cases （CMECduo）,and 0. 999999989 in trio cases （CMECtrio）. Conclusion All of the 12 loci in Investigator Argus X-- 12 system showed highly polymorphic in Guangdong Zhuang population. This study of allele frequencies would serve as a reference basic data for personal identification and paternity testing.