冠心病猝死心肌肌动蛋白缺失的免疫组织化学定量研究

应用免疫组织化学LSAB法和图像处理系统，对德国慕尼黑大学法医学研究所1994年尸检冠心病猝死和对照组病例（各15例）的心肌肌动蛋白缺失情况进行定量研究。发现冠心病猝死病例心肌纤维内有明显的肌动蛋白缺失，是大小不一，多发、散在分布；对照组无或仅局限性小灶状缺失。将检测结果定量分析比较，其差异非常显著，说明重度冠状动脉粥样硬化患者是否冠心病猝死，其心肌肌动蛋白缺失的范围和程度有明显差异，冠心病猝死和对照组病例心肌肌动蛋白缺失的差异具有诊断意义，而形态定量分析能较客观准确地反映心肌纤维内肌动蛋白缺失量的变化。

IMMUNOHISTOCHEMICAL AND QUANTITATIVE STUDY OF ACTIN DEPLETION IN MYOCARDIUM OF CASES DIED OF SUDDEN CORONARY HEART DISEASE

Actin depletion in myocardium from cases of sudden coronary death(SCD) and control group in Munich Institute of Forensic Medicine.Germany, was studied quantitatively by immunohistochemical technique (LSAB method ) and computer-assisted image processing. The results showed that there were marked depletion of actin in myocardium from cases of SCD. The actin depletions were multiple, disserninately and segmently distributed in cases of SCD,which they were sman area depletion in my ocardium of control group. The difference were statistically significant between the SCD and control group. The actin depletion has sorue significance in diagnosis of SCD. The morphometric study reflects the quantitative change of actin depletion in myocardium more objectively and accurately.