| 我国犬钩虫ITS及5.8S rDNA的克隆与序列分析 |
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| 引用本文： | 宋慧群,李宾,林瑞庆,朱兴全.我国犬钩虫ITS及5.8S rDNA的克隆与序列分析[J].中国兽医科学,2007,37(9):756-759. |
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| 作者姓名： | 宋慧群 李宾 林瑞庆 朱兴全 |
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| 作者单位： | 华南农业大学,兽医学院,广东,广州,510642 |
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| 摘 要： | 以从我国广东湛江犬小肠中采集的2条犬钩虫作为研究对象,用保守引物NC5及NC2扩增犬钩虫rDNA的ITS-1、5.8S及ITS-2片段,将PCR扩增出的片段纯化后克隆至pGEM-T Easy载体,重组质粒通过菌落PCR和酶切鉴定后,对阳性菌落进行序列测定并进行序列分析。结果显示,来自湛江的2条犬钩虫ITS及5.8S rDNA序列总长均为738 bp,其中ITS-1序列长为364 bp,5.8S序列长为153 bp,ITS-2序列长为221 bp;2个不同样品之间ITS序列存在多态性,ITS-1序列存在5个碱基的差异,ITS-2序列存在1个碱基的差异,5.8S rDNA序列无差异。研究结果为犬钩虫进一步的分类、鉴定和遗传变异研究奠定了基础。
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| 关 键 词： | 犬钩虫 内转录间隔区(ITS) 序列分析 |
| 文章编号： | 1673-4696(2007)09-0756-04 |
| 修稿时间： | 2007年7月9日 |
Cloning and sequence analysis of the ITS and 5.8S rDNA of Ancylostoma caninum in China |
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| SONG Hui-qun,LI Bin,LIN Rui-qing,ZHU Xing-quan.Cloning and sequence analysis of the ITS and 5.8S rDNA of Ancylostoma caninum in China[J].Veterinary Science in China,2007,37(9):756-759. |
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| Authors: | SONG Hui-qun LI Bin LIN Rui-qing ZHU Xing-quan |
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| Abstract: | The internal transcribed spacer(ITS) and 5.8S rDNA of Ancylostoma caninum isolated in Guangdong Province of China were amplified by PCR using a pair of conserved primers and the amplicons were cloned into pGEM-T Easy vector,respectively.The inserts were successfully sequenced,and the ITS of the two A.caninum samples was 738 bp in length.Sequence analysis revealed that the ITS-1,5.8S and ITS-2 rDNA of both A.caninum samples were 364 bp,153 bp and 221 bp in length,respectively,and polymorphism was found in the ITS-1 and ITS-2 rDNA sequences between the two A.caninum samples.The results provided a foundation for further studies of molecular identification and molecular genetics of A.caninum. |
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| Keywords: | 5 8S rDNA PCR |
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