| 生物检材中芬太尼和舒芬太尼的HPLC-MS/MS分析 |
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| 引用本文： | 董颖,邵永军,王瑞花.生物检材中芬太尼和舒芬太尼的HPLC-MS/MS分析[J].刑事技术,2010(5):23-25. |
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| 作者姓名： | 董颖 邵永军 王瑞花 |
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| 作者单位： | 1. 公安部物证鉴定中心,北京,100038
2. 江苏省无锡市滨湖公安分局刑事科学技术室,214072 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金计划重点项目，面上项目资助 |
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| 摘 要： | 目的建立血、肝组织中芬太尼和舒芬太尼的HPLC-MS/MS分析方法。方法采用Oasis（MCX固相萃取柱进行提取,以XTerraTMRP18柱（2.1mm×100mm,3.5μm）色谱柱分离,以乙腈∶5mmol/L醋酸铵水溶液（氨水调pH=9.5）（65∶35）为流动相,流速为0.2mL/min。结果血及肝组织添加样品的线性范围为10ng/mL～500ng/mL,最小检出限为0.1ng/mL。结论本方法准确、快速,可用于生物检材血、肝组织中芬太尼和舒芬太尼的定性定量分析。
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| 关 键 词： | 芬太尼 舒芬太尼 生物检材 固相萃取 HPLC-MS/MS |
Determination of Fentanyl and Sufentanil in biological samples by HPLC-MS/MS |
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| DONG Ying,SHAO Yong-jun,WANG Rui-hua.Determination of Fentanyl and Sufentanil in biological samples by HPLC-MS/MS[J].Forensic Science and Technology,2010(5):23-25. |
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| Authors: | DONG Ying SHAO Yong-jun WANG Rui-hua |
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| Institution: | (Institute of Forensic Science,Ministry of Public Security,Beijing 100038,China) |
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| Abstract: | Objective To establish HPLC-MS/MS method for the determination of Fentanyl and Sufentanil in biological samples. Methods Samples were extracted by solid-phase extraction. XTerraTM RP18 column （2.1mm×100mm,3.5μm） was used,and the mobile phase consisted of acetonitrile：ammonium acetate（5mmol/L,pH=9.5） （65：35）. The flow rate was 0.2 mL/min. Results There was a good linear relationship within the range of 10ng/mL～500ng/mL. The limit of detection was 0.1ng/mL.Conclusions The method is simple,accurate and can be used for the determination of Fentanyl and Sufentanil in biol-ogical samples. |
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| Keywords: | HPLC-MS/MS |
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