| 抗GC血清的制备及鉴定 |
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| 引用本文： | 侯一平,吴梅筠.抗GC血清的制备及鉴定[J].中国法医学杂志,1989(4). |
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| 作者姓名： | 侯一平 吴梅筠 |
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| 作者单位： | 华西医科大学法医血清学教研室，华西医科大学法医血清学教研室 |
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| 摘 要： | 作者用分离纯化的GC蛋白免疫新西兰兔制备了抗GC血清。鉴定结果表明,抗GC血清与进口商品抗GC血清特异性相同;与人血清其他蛋白没有交叉反应。琼脂双向扩散法测得抗血清效价为128;可检出GC蛋白的最低浓度为3.1μg/ml。免疫电泳法可测出入血清三种GC表型。
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| 关 键 词： | 型特异成分 维生素D结合蛋白 GC 抗GC血清 |
PREPARATION AND IDENTIFICATION OF ANTI-GC SERUM |
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| Hou Yiping Wu Meiyun.PREPARATION AND IDENTIFICATION OF ANTI-GC SERUM[J].Chinese Journal of Forensic Medicine,1989(4). |
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| Authors: | Hou Yiping Wu Meiyun |
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| Institution: | Hou Yiping Wu Meiyun Department of Forensic Serologg,West China University of Medical Sciences,Chengdu |
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| Abstract: | Anti-GC serum was successfully prepared in two New Zealand rabbits immunized with GC protein which was isolated and purified from GC2-2 serum previously in our laboratory. The results of identification showed that the specificity of the home made anti- GC serum and the commercial anti- GC serum (DAKOPATTS) were identical. The titer of the home made anti-GC serum was 128. Three common phenotypes, GC1-1, GC2-1 and GC2-2could be identified by immunoelectrophoresis with the home made anti-GC serum. The concentration of GC protein as low as 3.1 μg/ml could be detected by double immunodiffusion. In addition the anti-GC serum does not cross react with other human serum proteins. |
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| Keywords: | Group specific component Vitamin D binding protein GC protein Anti- GC serum |
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