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| 中国猪瘟兔化弱毒全基因组cDNA文库的构建和序列分析 | |
| 引用本文： | 胡建和,刘湘涛,庄淑珍,田宏,张彦明,谢庆阁.中国猪瘟兔化弱毒全基因组cDNA文库的构建和序列分析[J].中国兽医科学,2002,32(12):12-15. |
| 作者姓名： | 胡建和 刘湘涛 庄淑珍 田宏 张彦明 谢庆阁 |
| 作者单位： | 1. 中国农业科学院 兰州兽医研究所，甘肃 兰州 730046;西北农林科技大学 畜牧兽医学院，陕西 杨凌 712100 2. 中国农业科学院 兰州兽医研究所，甘肃 兰州 730046 3. 西北农林科技大学 畜牧兽医学院，陕西 杨凌 712100 |
| 基金项目： | 国家重点基础研究发展规划项目 (G1 9990 1 1 9) |
| 摘 要： | 根据已发表的猪瘟病毒 (CSFV)序列设计并合成了 2 6条覆盖全长基因组的套式和半套式PCR引物。应用RT PCR、NestedPCR和Half nestedPCR技术从试验感染的兔脾中成功地扩增出了各相应的cDNA重叠片段 ,分别克隆和测序 ,构建了C株全基因组cDNA文库。采用DNAstar软件对全基因组的核苷酸和氨基酸序列进行了同源性比较分析。CS FVC株全基因组cDNA文库的构建为进一步构建全长感染性cDNA奠定了基础 |
| 关 键 词： | 猪瘟病毒 C株 cDNA文库 构建 序列分析 |
| 文章编号： | 1000-6419(2002)12-0012-04 |
| 修稿时间： | 2002年10月22 |
Construction of genomic cDNA library and sequence analysis of the lapinized attenuated CSFV C strain |
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| HU Jian he ,LIUXiang tao ,ZHUANG Shu zhen ,TIAN Hong ,ZHANGYan ming ,XIEQing ge.Construction of genomic cDNA library and sequence analysis of the lapinized attenuated CSFV C strain[J].Veterinary Science in China,2002,32(12):12-15. | |
| Authors: | HU Jian he LIUXiang tao ZHUANG Shu zhen TIAN Hong ZHANGYan ming XIEQing ge |
| Institution: | HU Jian he 1,2,LIUXiang tao 1,ZHUANG Shu zhen 2,TIAN Hong 1,ZHANGYan ming 2,XIEQing ge 1 |
| Abstract: | Based on the published sequences of CSFV , 26 nested and half nested PCR primers covering the genome sequence of C strain were designed. Utilizing the RT PCR, nested and half nested PCR techniques, corresponding overlaping cDNA fragments were successfully amplified from total RNA extracted from spleens of experimentally infected rabbits. C strain of CSFV genomic cDNA library was constructed after cloning and sequencing of thesefragments. Comparing and analysing of homologies of nucleotideand amino acid sequences were performed by computer software DNAstar. Construction of genomic cDNA library makes the basis for construction of infectious full length cDNA of CSFV C strain. |
| Keywords: | classical swine fever virus C strain cDNA library construction sequence analysis |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |