Y-chromosomal short tandem repeats haplotyping from vaginal swabs using a chelating resin-based DNA extraction method and a dual-round polymerase chain reaction |
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Authors: | Iwasa Mineo Koyama Hiroyoshi Tsuchimochi Tsukasa Maeno Yoshitaka Isobe Ichiro Seko-Nakamura Yoshimi Monma-Ohtaki Jun Matsumoto Tomohiro Nagao Masataka |
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Institution: | Department of Forensic Medical Science, Nagoya City University Graduate School of Medical Sciences, Kawasumi 1, Mizuho-ku, Japan. nagaom@med.nagoya-cu.ac.jp |
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Abstract: | Reported are 2 autopsy cases in which Y-chromosomal microsatellite short tandem repeats DYS19, DYS389I and II, DYS390, and DYS393 could be haplotyped with vaginal swabs by using a Chelex 100-based DNA extraction method and dual-round polymerase chain reaction. The extraction of DNA from vaginal swabs by using this method was as efficient or more efficient than using proteinase K and phenol-chloroform extraction or the alkaline lysis methods. Y-chromosomal microsatellite short tandem repeats haplotyping based on the dual-round polymerase chain reaction method provided genotypes from all the loci determined. Although amplification of Y-chromosomal microsatellite short tandem repeats loci is not directly involved in the existence of spermatozoa, it is considerably advantageous for male individualization from body fluid mixture stains in criminal cases. |
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