对Cofiler体系在亲子鉴定中应用价值的研究

将商品化 Profiler Plus和 Cofiler扩增检测试剂盒用于亲子鉴定案例和无关个体血样的分析。 186例亲子鉴定案中,母－子－假设父三联体 149例,假设父－子二联体 37例。在 149例含双亲和孩子的鉴定中,有 127例认定存在亲生血缘关系,其 RCP值平均为 99.98％;其余 22例排除了被检父为孩子生父,排除机率为 14.77％,排除指标平均为 6.1个。在 37例含单亲及孩子的鉴定中,有 23例认定存在亲生血缘关系,其 RCP值平均为 99.92％;其余 14例排除被检父为孩子生父,排除机率为 37.84％,平均有 5个不吻合的 STR位点出现。若特意省去三人组合案例中的母亲,将其作为单亲案件进行统计,仅检测 Profiler Plus体系的 9个 STR位点时,则 RCP值平均为 99.1959％,案例中 21.51％将不能得出明确的结论。同时检测 Profiler Plus和 Cofiler体系共 13个 STR位点,则案例中 97.31％能排除或认定生父,案例中 2.69％通过补充母亲样本能排除或认定生父。 Cofiler体系中 6个 STR位点的累积排除率达 0.9825。上述数据表明, Cofiler试剂盒在单亲鉴定案件中有着重要的应用价值。

Usefulness of Cofiler PCR amplification kit in parentage testing

Parentage testing was performed by using multiple PCR and automated genescan and genotype analysis. Parentage was excluded in 36 cases,including 22 of 149 paternity trios and 14 of 37 paternity duos. In the 22 trios and 14 duos in which paternity was excluded, a mean of 6.1 and 5 incompatible STR markers were noted. The application value of six loci which included in the Cofiler amplification kit was showed here. The discrimination power ranged from 0.8023 for TPOX to 0.9203 for D16S539. The cumulative power of exclusion was provided as 0.9825. Three mutation were found (one each at D3S1358,TPOX,CSF1PO). For most nonexcluded paternity duos, only when the Cofiler amplification kit was used in conjunction with Profiler Plus kit, the RCP value was acceptable. The data showed that the Cofiler kit could play an important role in cases of paternity duos.