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| 小鼠死后骨骼肌、心、肝、肾、脑细胞核中DNA的变化规律 | |
| 引用本文： | 郑吉龙,李晓娜,张晓东,牛青山.小鼠死后骨骼肌、心、肝、肾、脑细胞核中DNA的变化规律[J].法医学杂志,2010,26(3):161-164. |
| 作者姓名： | 郑吉龙 李晓娜 张晓东 牛青山 |
| 作者单位： | 1. 中国医科大学法医学院,辽宁,沈阳,110001;中国刑事警察学院,法医系,辽宁,沈阳,110035 2. 中国医科大学基础医学院,辽宁,沈阳,110001 3. 中国刑事警察学院,法医系,辽宁,沈阳,110035 |
| 基金项目： | 公安部应用创新计划项目，辽宁省高等学校科研项目 |
| 摘 要： | 目的 研究小鼠器官细胞核中DNA降解变化与死亡时间的关系,为法医学推断死亡时间提供一种新方法.方法 应用单细胞凝胶电泳技术结合荧光显微镜和计算机图像分析技术,测定小鼠在死后72h内不同时间点骨骼肌、心、肝、肾、脑细胞核头DNA含量、尾DNA含量、头半径、尾长度、尾矩、Olive矩、头面积、尾面积8项参数的变化值.结果 在小鼠死亡72h内,彗星尾DNA含量、尾长度、尾矩、Olive矩、尾面积都呈增加趋势,头DNA舍量、头半径、头面积均呈下降趋势.计算出体现DNA降解趋势的二项式回归方程(P<0.001)和多元回归方程(P<0.0001).结论 本研究提供的各组回归方程,可为法医学推断死后经过时间提供一种新的方法. |
| 关 键 词： | 法医病理学 细胞核 DNA降解 死亡时间推断 小鼠 |
DNA Degradation in Nucleolus of Skeletal Muscle, Heart, Liver, Kidney and Brain in Mice after Death |
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| ZHENG Ji-long,LI Xiao-na,ZHANG Xiao-dong,NIU Qing-shan.DNA Degradation in Nucleolus of Skeletal Muscle, Heart, Liver, Kidney and Brain in Mice after Death[J].Journal of Forensic Medicine,2010,26(3):161-164. | |
| Authors: | ZHENG Ji-long LI Xiao-na ZHANG Xiao-dong NIU Qing-shan |
| Institution: | ZHENG Ji-long1,2,LI Xiao-na3,ZHANG Xiao-dong2,NIU Qing-shan2(1.School of Forensic Medicine,China Medical University,Shenyang 110001,China,2.Department of Forensic Medicine,China Criminal Police University,Shenyang 110035,3.College of Basic Medical Science,China) |
| Abstract: | Objective To study the change of DNA degradation in nucleolus of mice organs and its relationship with the postmortem interval,and to investigate a new accurate method to estimate the postmortem interval.Methods Eight parameters of cell nuclei were chosen,including the head DNA level,the tail DNA level,the head radius,the tail length,the tail moment,the Olive moment,the head area and the tail area.The changes of DNA degradation were analyzed in skeletal muscle,myocardium,liver,kidney and brain in mice at di... |
| Keywords: | forensic pathology cell nucleus DNA degradation postmortem interval estimation mice |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |