Group-specific component imaging from trace material using isoelectric focussing and electroblotting |
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Authors: | U K?mpfe A Correns H Schr?der P Otremba G Geserick |
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Affiliation: | Institut für Gerichtliche Medizin, Humboldt-Universit?t zu Berlin. |
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Abstract: | GC-subtyping was carried out on blood stains, that had been made on glass and stored under room temperature-conditions. Using isoelectric focusing in polyacrylamide gel, followed by transfer to nitrocellulose membrane by semi-dry-electroblotting and detection with enzyme-linked antibody complex the GC-detection was possible even after 64 days. Methodical problems are discussed. |
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