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| 斑点免疫金渗滤法检测猪生殖与呼吸综合征抗体的研究 | |
| 引用本文： | 孔繁德,黄印尧,王生育,张春安,林开正,陆承平.斑点免疫金渗滤法检测猪生殖与呼吸综合征抗体的研究[J].中国兽医科学,2005,35(1):36-40. |
| 作者姓名： | 孔繁德 黄印尧 王生育 张春安 林开正 陆承平 |
| 作者单位： | 1. 南京农业大学,动物医学院,江苏,南京,210095;厦门出入境检验检疫局,福建,厦门,361012 2. 厦门出入境检验检疫局,福建,厦门,361012 3. 厦门市波生生物技术有限公司,福建,厦门,361005 4. 厦门市湖里兽医站,福建,厦门,361012 5. 南京农业大学,动物医学院,江苏,南京,210095 |
| 基金项目： | 厦门市湖里区科技项目(2002 05 01) |
| 摘 要： | 应用提纯的猪生殖与呼吸综合征病毒(PRRSV)抗原包被硝酸纤维素膜,然后用胶体金标记的金黄色葡萄球菌A蛋白(SPA)建立了检测PRRS抗体水平的斑点免疫金渗滤法(DIGFA)。该法通过胶体金标记SPA直接显色,阳性者出现红色斑点,整个试验过程仅需5min即可判断结果;与猪瘟、猪伪狂犬病、猪细小病毒病阳性血清不发生交叉反应;纯化PRRSV抗原的最低检测量为0.0553mg mL,即55.3ng 点。对200份猪血清用该法与ELISA同时进行PRRS抗体检测,两种方法的符合率达98%。 |
| 关 键 词： | 斑点免疫金渗滤法 猪生殖与呼吸综合征 抗体 检测 |
| 文章编号： | 1000-6419(2005)01-0036-05 |
| 修稿时间： | 2004年7月9日 |
Detection of antibody against PRRSV by dot immunogold filtration assay |
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| KONG Fan-de.Detection of antibody against PRRSV by dot immunogold filtration assay[J].Veterinary Science in China,2005,35(1):36-40. | |
| Authors: | KONG Fan-de |
| Institution: | KONG Fan-de~ |
| Abstract: | A dot immunogold filtration assay(DIGFA) was developed by coating antigen of PRRSV on nitrocellulose membrane and labeling staphylococcal protein A(SPA) marked with colloidal gold to establish a rapid and simple method for detecting antibody to PRRSV. The color of the positive dot was so bright that the result could be determined easily. The whole procedure of the DIGFA could be finished within 5 minutes. The method has no cross-reaction with positive sera with SFV,PRV,PPV and JEV respectively. (0.055 3) mg/mL PRRSV antigen and above can be detected out. The coincidence rate between the results by ELISA and DIGFA was 98% for antibody to PRRSV. |
| Keywords: | dot immunogold filtration assay(DIGFA) PRRS antibody detection |
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