2004-2010年浙江省及其周边地区PRRSV GP5基因的分子进化与变异分析

用RT-PCR方法对分离自浙江省及其周边地区部分猪场的50个猪繁殖与呼吸综合征病毒(PRRSV)毒株和3个疫苗毒株的GP5基因进行了扩增、克隆和序列分析,并与国内外的代表性毒株进行了序列比对。结果表明,2004-2010年检测的PRRSV毒株均属于美洲型,大部分毒株属于亚群1,各毒株间的核苷酸和氨基酸同源性分别在81.6%～100%和72.1%～99.5%之间,浙江省及其周边地区亚群1毒株与我国最早分离到的美洲型毒株CH-1a的核苷酸和氨基酸同源性分别为84.5%～95.7%和78.6%～95.0%,与疫苗株的核苷酸和氨基酸的同源性分别为88.1%～99.2%和85.4%～99.0%。糖基化位点以及抗原表位都有一定程度上的变异。

Genetic variation and phylogenetic analysis of glycoprotein 5(GP5) gene of porcine reproductive and respiratory syndrome viruses in Zhejiang Province and its surrounding areas from 2004 to 2010

The GP5 gene was amplified and sequenced from 3 porcine reproductive and respiratory syndrome virus(PRRSV) vaccine strains and 50 tissue samples of pigs suspected PRRSV infection in Zhejiang Province and its surrounding areas. Phylogenetic analysis indicated that all isolates from the region from 2004 to 2010 belonged to subgroup 1 of American type which shared 81.6%-100% identities in nucleotide level and 72.1%-99.5% in amino acid level. And they had 84.5%-95.7% of nucleotide identity and 85.4%-99.0% of am...