| 牛病毒性腹泻弱毒活疫苗的安全性和免疫保护效果研究 |
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| 引用本文： | 张淑琴,郭利,张亭亭,吴永旺,武华.牛病毒性腹泻弱毒活疫苗的安全性和免疫保护效果研究[J].中国兽医科学,2011(10). |
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| 作者姓名： | 张淑琴 郭利 张亭亭 吴永旺 武华 |
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| 作者单位： | 中国农业科学院特产研究所;北京必威安泰生物科技有限公司; |
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| 基金项目： | 牛羊重大疫病防控技术研究与产业化项目(200803018); 动物疫苗耐热保护剂及冻干技术研究与开发项目(2009BADB4B02) |
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| 摘 要： | 为评价牛病毒性腹泻病毒弱毒株的安全性及其免疫保护效果,将致细胞病变型牛病毒性腹泻病毒(BVDV)驯化培养,接种MDBK细胞,优化病毒增殖条件,并将其免疫牛,评价其安全性及免疫保护效果。结果显示,优化后的病毒效价可达107.5TCID50/mL。动物试验结果显示,1月龄牛接种该弱毒株后,体温正常,白细胞数量没有下降,无任何临床异常表现。免疫牛用BVDV强毒株进行攻毒;结果表明,免疫保护效果良好。表明该毒株可以作为弱毒活疫苗研究的候选毒株。
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| 关 键 词： | 牛病毒性腹泻病毒 弱毒活疫苗 安全性 免疫 保护效果 |
Research on the safety and protective efficacy of a live attenuated bovine viral diarrhea virus(BVDV) vaccine |
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| ZHANG Shu-qin,GUO Li,ZHANG Ting-ting,WU Yong-wang,WU Hua.Research on the safety and protective efficacy of a live attenuated bovine viral diarrhea virus(BVDV) vaccine[J].Veterinary Science in China,2011(10). |
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| Authors: | ZHANG Shu-qin GUO Li ZHANG Ting-ting WU Yong-wang WU Hua |
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| Institution: | ZHANG Shu-qin1,GUO Li1,ZHANG Ting-ting2,WU Yong-wang2,WU Hua1(1.Institute of Special Economic Animal and Plant Sciences,Chinese Academy of Agricultural Sciences,Jilin 132109,China,2.Beijing Bigvet Biotech Co.,Ltd.,Beijing 100176,China) |
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| Abstract: | The cytopathogenic bovine viral diarrhea virus(BVDV) was domesticated and habituated to MDBK cells preserved in our laboratory.The high virus titer of 107.5 TCID50/mL was achieved by optimized replication conditions,and the safety and protective efficacy(immunogenicity) of the BVDV was tested in host animal as well.The evaluation results showed that the one-month-calves vaccinated with the low virulent strain did not show any clinical diseases,with the body temperature and the white blood cell count(WBC) be... |
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| Keywords: | bovine viral diarrhea virus(BVDV) immunization safety protective efficacy |
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