高效液相色谱法测定血浆中雷公藤甲素和雷公藤酮

目的　建立高效液相色谱法定量检测人血浆中雷公藤甲素和雷公藤酮的分析方法。方法　以Oasis HLB固相萃取柱对样品进行提取 ,应用HPLC色谱法二极管阵列检测器测定。结果　该方法的回收率高于 80 % ,线性范围在 10～ 10 0 0ng/ml ,经该方法测得雷公藤甲素的最小检出限为 3 0ng/ml,雷公藤酮的最小检出限为 4 5ng/ml(S/N≥ 3 )。结论　该方法快速灵敏、准确 ,适用于雷公藤中毒的法医学检验。

Determination of Triptolide and Triptonide in plasma by HPLC

Objective To establish a method for quantitative determination of triptolide and triptonide in human plasma samples by HPLC.Methods The samples were extracted with Oasis HLB solid phase extraction cartridge and analyzed with reserved phase HPLC coupled with diode array detection.Results Recoveries rates obtained from spiked plasma for the two diterpenoid were higher than 80%.The linearity ranged from 10 ng/ml to 1 000 ng/ml.Detection limits of the method were 3.0ng/ml for triptolide and 4.5ng/ml for triptonide.Conclusion The method is fast and accurate,which is applicable to determination of diterpenoid in cases of suspected poisoning of Triptergium wilfordii Hook.f. in forensic practice.