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| 利用荧光AFLP技术检测罂粟DNA多态性 | |
| 引用本文： | 路帆,程宝文,李虹,洪俊杰,孙海燕,赵文嵩,杨红梅,李进,赵郁荣.利用荧光AFLP技术检测罂粟DNA多态性[J].法医学杂志,2008,24(4):262-264,267. |
| 作者姓名： | 路帆 程宝文 李虹 洪俊杰 孙海燕 赵文嵩 杨红梅 李进 赵郁荣 |
| 作者单位： | 1. 云南省公安厅刑事科学技术研究所,云南,昆明,650021 2. 昆明明技科技有限公司,云南,昆明,650023 3. 普洱市公安局刑事科学技术研究所,云南,普洱,653400 4. 临沧市公安局刑事科学技术研究所,云南,临沧,653700 |
| 摘 要： | 目的 利用荧光AFLP检测技术检测罂粟植物DNA多态性。方法 用Axygen公司的AxyPrep DNA试剂盒提取了12株产于缅甸和中国云南省昆明市宜良县罂粟植株的DNA，用Eco RI和Mse I对总DNA进行酶切。连接人工接头，预扩增和选择性扩增，其产物在CEQ8000遗传分析系统上检测。结果 64对选择性扩增引物中8对引物能得到20条以上的扩增片断，具有高度多态性。结论 荧光AFLP技术可用于罂粟DNA多态性的检测。 |
| 关 键 词： | 法医学 植物学 罂粟 扩增片段长度多态性 |
DNA Polymorphism Detection of Papaver Somniferum L Using Fluorescent Amplifled Fragment Length Polymorphism |
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| LU Fan,CHENG Bao-wen,LI Hong,HONG Jun-jie,SUN Hai-yan,ZHAO Wen-song,YANG Hong-mei,LI Jin,ZHAO Yu-rong.DNA Polymorphism Detection of Papaver Somniferum L Using Fluorescent Amplifled Fragment Length Polymorphism[J].Journal of Forensic Medicine,2008,24(4):262-264,267. | |
| Authors: | LU Fan CHENG Bao-wen LI Hong HONG Jun-jie SUN Hai-yan ZHAO Wen-song YANG Hong-mei LI Jin ZHAO Yu-rong |
| Institution: | LU Fan, CHENG Bao-wen, LI Hong, HONG Jun-jie, SUN Hal-yan, ZHAO Wen-song, YANG Hong- mei, LI Jin, ZHAO Yu-rong (1. Institute of Criminal Science and Technology, Yunnan Province Police Bureau, Kunming 650021, China; 2. Kunming Mingji Science and Technology Co., Ltd, Kunming 650023,China; 3. Institute of Criminal Science and Technology, Pu＇er Public Security Bureau, Pu＇er 653400,China; 4. Institute of Criminal Science and Technology, LingCang Public Security Bureau, Lingcang 653700, China) |
| Abstract: | Objective To detect DNA polymorphism of Papaver somniferum L using fluorescent Amplified Fragment Length Polymorphism.Methods Genomic DNA was isolated using the AxyPrep DNA Kit,double-digested by two restrictional endonucleases(Eco RI and Mse I) and ligated to oligonucleotide adapters.After Pre-amplification and selective amplification,the DNA fragments were separated by capillary electrophoresis using the CEQ8000 DNA Fragment Analyzer.Results More than 20 fragments of highly polymorphic products were obtained by 8 pairs of primer from 64 selective amplifying primer pairs.Conclusion The fluorescent AFLP technique can be used to detect the DNA polymorphism of Papaver somniferum. |
| Keywords: | forensic medicine botany papaver somniferum L amplified fragment length polymorphism |
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