| RGD基序邻近氨基酸突变对口蹄疫病毒O/JC/2010株毒力的影响 |
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| 引用本文： | 况文东,白兴文,李平花,祁国财,包慧芳,卢曾军,孙普,谢宝霞,郝晓芳,刘在新.RGD基序邻近氨基酸突变对口蹄疫病毒O/JC/2010株毒力的影响[J].中国兽医科学,2012(5):441-447. |
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| 作者姓名： | 况文东 白兴文 李平花 祁国财 包慧芳 卢曾军 孙普 谢宝霞 郝晓芳 刘在新 |
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| 作者单位： | 中国农业科学院 兰州兽医研究所 家畜疫病病原生物学国家重点实验室 农业部畜禽病毒学重点实验室 国家口蹄疫参考实验室 |
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| 基金项目： | 公益性行业(农业)科研专项(201103008) |
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| 摘 要： | 为了研究RGD基序邻近氨基酸位点对口蹄疫病毒毒力的影响,利用O/JC/2010株病毒的基因组全长cDNA分子克隆,置换O/HN/93株病毒的VP1基因,拯救获得了1株嵌合病毒(pBlue-HJ);除此之外,在O/JC/2010VP1的3个氨基酸位点进行突变P142T、A152D、Q153P,分为4组,拯救出4株突变病毒:pBlue-HJ(142)、pBlue-HJ(142+152)、pBlue-HJ(152+153)、pBlue-HJ(142+152+153)。比较这5株病毒的LD50和TCID50特性,发现P142T、A152D可以增强口蹄疫病毒对BHK-21细胞和乳鼠的致病力,而Q153P则相反。该试验证明RGD基序附近的非保守氨基酸位点对口蹄疫病毒的毒力存在影响,为进一步阐明口蹄疫病毒的分子致病机制奠定了基础。
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| 关 键 词： | 口蹄疫病毒 G-H环 RGD基序 毒力 |
Role of the amino acids adjacent to RGD motif in the virulence of foot-and-mouth disease virus strain O/JC/2010 |
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| KUANG Wen-dong,BAI Xing-wen,LI Ping-hua,QI Guo-cai,BAO Hui-fang,LU Zeng-jun,SUN Pu,XIE Bao-xia,HAO Xiao-fang,LIU Zai-xin.Role of the amino acids adjacent to RGD motif in the virulence of foot-and-mouth disease virus strain O/JC/2010[J].Veterinary Science in China,2012(5):441-447. |
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| Authors: | KUANG Wen-dong BAI Xing-wen LI Ping-hua QI Guo-cai BAO Hui-fang LU Zeng-jun SUN Pu XIE Bao-xia HAO Xiao-fang LIU Zai-xin |
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| Institution: | (State Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Animal Virology of the Ministry of Agriculture/National Foot-and-Mouth Disease Reference Laboratory/Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China) |
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| Abstract: | In order to study the role of neighboring amino acids of RGD motif in the virulence of foot-and-mouth disease virus(FMDV),the VP1 coding region of O/JC/2010 strain was used to substituted for counterpart of genetically engineered virus O/HN/93.The chimeric FMDV strain(named pBlue-HJ) was got by reverse genetic technology.Besides,four chimeric FMDV strains pBlue-HJ(142),pBlue-HJ(142+152),pBlue-HJ(152+153),pBlue-HJ(142+152+153) were constructed through a serious of mutations(P142T,A152D and Q153P) in VP1.Mutations P142T and A152D were beneficial to virulence increasing in the BHK-21 cell and sucking mouse,nevertheless,Q153P went against virulence increasing.The results showed that the nonconservative amino acid sites adjacent to RGD motif could affect the virulence of FMDV.The study lays a foundation for further study of molecular pathogenesis of FMDV. |
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| Keywords: | foot-and-mouth disease virus G-H loop RGD motif virulence |
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