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| RT-PCR与兔体交叉试验检测猪瘟病毒感染的相关性研究 | |
| 引用本文： | 刘建柱,崔玉东,邢华伟,朱战波,朴范泽. RT-PCR与兔体交叉试验检测猪瘟病毒感染的相关性研究[J]. 中国兽医科学, 2003, 33(11): 16-21 |
| 作者姓名： | 刘建柱 崔玉东 邢华伟 朱战波 朴范泽 |
| 作者单位： | 1. 黑龙江八一农垦大学,生命科学技术学院,黑龙江,大庆,163311 2. 黑龙江八一农垦大学,生命科学技术学院,黑龙江,大庆,163311;黑龙江八一农垦大学,动物科技学院,黑龙江,大庆,163311 3. 黑龙江八一农垦大学,动物科技学院,黑龙江,大庆,163311 |
| 基金项目： | 黑龙江省科委基金资助项目 (GB0 1B10 5 6 0 2 ) |
| 摘 要： | 对猪瘟RT PCR诊断方法与兔体交叉试验诊断方法的相关性进行了研究。通过计算机软件设计了猪瘟病毒E2基因保守区的 1对引物 ,建立了猪瘟病毒RT PCR检测方法 ,同时应用兔体交叉试验进行对比 ,对 7份临床送检病例进行了检测。结果表明 ,二者的符合率为 10 0 % ,但RT PCR可大大降低检测所用的时间 ,更便于猪瘟的快速诊断。 |
| 关 键 词： | RT-PCR 兔体交叉试验 猪瘟病毒 |
| 文章编号： | 1000-6419(2003)11-0016-06 |
| 修稿时间： | 2003-08-28 |
Study on relationship between RT-PCR and rabbit-cross test for detection of classical swine fever virus |
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| LIU Jian-zhu,CUI Yu-dong,,XING Hua-wei,ZHU Zhan-bo,PIAO Fan-ze. Study on relationship between RT-PCR and rabbit-cross test for detection of classical swine fever virus[J]. Chinese Veterinary Science, 2003, 33(11): 16-21 | |
| Authors: | LIU Jian-zhu CUI Yu-dong XING Hua-wei ZHU Zhan-bo PIAO Fan-ze |
| Affiliation: | LIU Jian-zhu~1,CUI Yu-dong~1,2,XING Hua-wei~2,ZHU Zhan-bo~2,PIAO Fan-ze~2 |
| Abstract: | Relationship between RT-PCR and rabbit-cross test for detection of classical swine fever virus (CSFV) was studied in this experiment. One set of specific primers were designed according to the conserved sequence of E2 gene of CSFV in GenBank and a standard RT-PCR was founded to detect the infection of CSFV. Compared with rabbit-cross test, 7 samples from pigs suspected infection with CSFV were detected with RT-PCR. It was indicated that the results from the detection of CSFV by RT-PCR coinside completely with that from the detection with rabbit-cross test, and that RT-PCR detection can save plenty of time. |
| Keywords: | RT-PCR rabbit-cross test CSFV |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |