SD大鼠肢体挫伤后血清生化指标变化及其法医学意义

目的观察大鼠肢体挫伤后血清生化指标的变化特征，分析其法医学意义。方法实验选用SD大鼠，用自由落体装置分别击打大鼠右侧大腿根部，按垂直打击高度控制力度，设置为对照组、轻度打击组、中度打击组和重度打击组等4组。取实验鼠血清检测肌酸激酶（CK）、肌酸激酶同功酶（CK-Mb）、乳酸脱氢酶（LDH）、肌酐（Cr）、尿素氮（BUN）、α-羟丁酸脱氧酶（HBDH）及天门冬氨酸转移酶（AST）的变化，使用SPSS12．0软件分析各指标变化趋势。结果7种血清生化指标AST、CK、CK—MB、HBDH、BUN、Cr随打击力度增加而上升，其中轻、中、重打击组作为整体损伤组与对照组比较有显著性差异，AST、CK、CK—Mb在打击组间比较具有显著性差异。打击组肢体周径与未打击组肢体周径比较有明显变化，且与打击力度呈线性相关。结论肢体软组织挫伤造成相关血清生化指标的显著变化，提示了发生早期心功能损害的部分机制，并且其损伤程度与打击力度呈现线性关系。

Medico-legal evaluation followed muscular contusion based on the biochemical changes in SD rats' serum

Objective To observe the variations of biochemical indicators in serum followed the different contusion on soft tissues and also analyze their significance in forensic practice.Methods The SD rats were selected as experimental animal as well as were randomly divided into four groups: sham control,mild,medium and strong force depending on the height of strike falling in anesthesia state.Sacrificed the rats at the same time point and harvested the blood.Serumal creatine kinase(CK),isoenzyme of creatine kinase(CK-MB),lactate dehydrogenase(LDH),creatinine(Cr),urea nitrogen(UN) α-hydroxybutyrate dehydrogenase(HBDH) and aspartic acid transferase(AST) were tested.The data were analyzed quantitatively with SPSS 12.0.Results The AST,CK,CK-Mb,HBDH,UN and Cr all increased accompanied with higher scale of strike.The statistics showed that a dramatically difference existed between the overall traumatic groups(including to the mild,medium and strong) and the control.The AST,CK and CK-MB showed significant differences in 3 experimental groups.Meanwhile,the perimeter of the traumatic limbs was a good marker for the power of the strike.Conclusion The contusion not only appeared in the local areas but also cause the myocardial damage in the period of early trauma.