|
|||||
|
|
Use of “AnyDirect PCR buffer” for PCR amplification of washed bloodstains: A case report |
|
| Authors: | A. Barbaro P. Cormaci A. Barbaro |
| Affiliation: | a Department of Forensic Genetics, SIMEF-89128 Reggio Calabria, Italy b Department of Chemistry, SIMEF-89128 Reggio Calabria, Italy c SIMEF Director-89128 Reggio Calabria, Italy |
| Abstract: | AnyDirect PCR buffer (BioQuest) permits to perform direct PCR from different kinds of forensic samples (blood, saliva, sperm, etc.) without any DNA purification step.This is very useful in particular when working with small quantity of sample since it avoids the risk of loosing sample step by step as often happens with traditional DNA extraction procedures.In the present casework we analyzed some bloodstains found by luminol test onto washed clothes and linen: all samples were analyzed either using the AmpFlSTRs Identifiler kit (Applied Biosystems). |
| Keywords: | Washed bloodstains Direct PCR MiniSTRs |
| 本文献已被 ScienceDirect 等数据库收录！ | |