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Single primer extension (SPEX) amplification to accurately genotype highly damaged DNA templates |
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| Authors: | Paul Brotherton Phillip Endicott Ross Barnett Alan Cooper |
| Institution: | a Australian Centre for Ancient DNA, School of Earth and Environmental Sciences, University of Adelaide, Adelaide, SA 5005, Australia b Henry Wellcome Ancient Biomolecules Centre, University of Oxford, Department of Zoology, South Parks Road, Oxford OX1 3PS, UK c School of Animal and Microbial Sciences, University of Reading, Reading RG6 6AG, UK d Department of Archaeology, University of Durham, South Road, Durham DH1 3L, UK e National Institute of Toxicology and Forensic Sciences, Canary Islands delegation, 38320, Tenerife, Spain |
| Abstract: | High levels of non-authentic sequence data can be generated by traditional PCR-based methodologies when DNA is damaged, template numbers are small and/or the target amplification size too large. We therefore present an alternate methodology based on single primer extension (SPEX) amplification; that places no pre-defined size constraints on amplification and interacts with only one of the DNA strands at the target locus. |
| Keywords: | DNA damage Genotyping Low copy number DNA SNPs |
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